LIFE CYCLE, SIZE REDUCTION PATTERNS, AND ULTRASTRUCTURE OF THE PENNATE PLANKTONIC DIATOM PSEUDO‐NITZSCHIA DELICATISSIMA (BACILLARIOPHYCEAE) 1

By A. Amato, L. Orsini, D. D’Alelio, Marina Montresor. 2005

132 citations recorded by the source corpus.

Pseudo‐nitzschia delicatissima (Cleve) Heiden is a very common pennate planktonic diatom found in temperate marine waters, where it is often responsible for blooms. Recently, three distinct internal transcribed spacer types have been recorded during a P. delicatissima bloom in the Gulf of Naples (Mediterranean Sea, Italy), which suggests the existence of cryptic diversity. We carried out mating experiments with clonal strains belonging to the most abundant internal transcribed spacer type. Pseudo‐nitzschia delicatissima is heterothallic and produces two functional anisogametes per gametangium. The elongated auxospore possesses a transverse and a longitudinal perizonium. The sexual phase was observed to occur over a wide size spectrum, spanning 19–80 μm and corresponding to almost the whole range of cell length observed for P. delicatissima. We also investigated cell morphology, valve ultrastructure and morphometry of parental, F1‐generation strains, and the progeny of crosses between parental and F1 strains. Although ultrastructural features match those described for P. delicatissima, variability in cell shape was recorded in the largest cells of the F1 generation as well as in valves with an abnormal arrangement of poroids. As many other diatoms, P. delicatissima undergoes size reduction over its life cycle, and cells of different size showed differences in growth rates and the amount of size reduction per cell cycle. Cells between 60 and 30 μm in length showed the fastest growth and the slowest rates of size reduction per generation. In culture, P. delicatissima cells can decrease to 8 μm in length; however, such small cells (≤30 μm) are not recorded in the sea, and this raises interesting questions about the factors that control their survival in the natural environment.

Subfields: Biomaterials, Molecular Biology, Oceanography

View Paper: https://www.semanticscholar.org/paper/df848901fbf57a2ccb5adbbd039e7c74329e2eda

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Citations in the corpus

Pseudo-nitzschia physiological ecology, phylogeny, toxicity, monitoring and impacts on ecosystem health (2012, background): “…Davidovich and Bates, 1998), P. fraudulenta (Chepurnov et al., 2004), P. pungens (Chepurnov et al., 2005; Casteleyn et al., 2008), P. delicatissima (Amato et al., 2005, 2007; Kaczmarska et al., 2008; Quijano-Scheggia et al., 2009b), P. pseudodelicatissima sensu stricto (Amato et al., 2007), P.…”
Unveiling the mysteries of phytoplankton life cycles: patterns and opportunities behind complexity (2011, background): “The maximum growth rate of diatoms can change during their life cycle (Amato et al., 2005; von Dassow et al., 2006) and discarding of parental frustules during sex might, at times, be a major source of sinking silica (Crawford, 1995) with a large amount of associated particulate organic carbon.”
Proposals for a terminology for diatom sexual reproduction, auxospores and resting stages (2013, background): “Peragallo (Amato et al. 2005), some clones of Tabularia fasciculata (Davidovich et al.”
Trait-Based Community Ecology of Phytoplankton (2008, background): “In diatoms, sexual reproduction is also a means to restore cell size, because asexual reproduction leads to miniaturization (Amato et al. 2005, Mann 1993).”
Pseudo-nitzschia (Bacillariophyceae) species, domoic acid and amnesic shellfish poisoning: revisiting previous paradigms (2012, background): “The cause of ‘lobed’, ‘undulate’ or ‘sickle-shaped’ cell deformities (Mengelt & Prézelin 2002), most often found in older cultures (Amato et al. 2005; Li et al. 2005; Hagström et al. 2010) but also reported in some field samples (discussed in Bates et al. 1998), is still not fully explained.”
The time for sex: A biennial life cycle in a marine planktonic diatom (2010, methodology): “This trend was inferred from experimental data on P. multistriata (D’Alelio et al. 2009b) and Pseudo-nitzschia delicatissima (Amato et al. 2005).”
Coupled changes in the cell morphology and elemental (C, N, and Si) composition of the pennate diatom Pseudo‐nitzschia due to iron deficiency (2007, background): “This differs from the diminution process, which results in a more pronounced decrease in the valve apical length dimension and a reduction in the valve aspect ratio (Amato et al. 2005).”
INTER‐ AND INTRASPECIFIC VARIATION OF THE PSEUDO‐NITZSCHIA DELICATISSIMA COMPLEX (BACILLARIOPHYCEAE) ILLUSTRATED BY RRNA PROBES, MORPHOLOGICAL DATA AND PHYLOGENETIC ANALYSES 1 (2006, background): “A recent study has reported on mating between strains of P. delicatissima from Gulf of Naples (Amato et al. 2005).”
Morphology, physiology, molecular phylogeny and sexual compatibility of the cryptic Pseudo-nitzschia delicatissima complex (Bacillariophyta), including the description of P. arenysensis sp. nov. (2009, background): “The morphological abnormalities found in some of the F2 strains correspond to the variations in the F1 generation reported by Amato et al. (2005).”