Conformational analysis of complex protein states by hydrogen/deuterium exchange mass spectrometry (HDX-MS): Challenges and emerging solutions

By Esben Trabjerg, Z. Nazari, K. Rand. Trends in Analytical Chemistry (TrAC), 2018

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Abstract Hydrogen/deuterium exchange mass spectrometry (HDX-MS) has within the last 25 years evolved to become a well-established technique for analysing the solution-phase conformational dynamics and molecular interactions of proteins, attributes that can be difficult to access by other methods for structural analysis. This review presents the latest development within HDX-MS with a special focus on how improvements in instrumentation, methodology and data analysis have expanded the capability of HDX-MS to analyse complex and hitherto elusive protein states. Such protein states include large or multi-protein complexes, lipid-associated or integral membrane proteins, proteins with highly dynamic regions, disulfide-bonded proteins, heavily glycosylated proteins and proteins in formulations.

Subfields: Molecular Biology, Spectroscopy

View Paper: https://www.semanticscholar.org/paper/dd0790106bdcf23048ec51912b8bc3a67a92d5cc