Assessing temporal and spatial variability in Pfiesteria piscicida distributions using molecular probing techniques

By KJ Coyne, DA Hutchins, C. Hare, Scott Cary. 2001

120 citations recorded by the source corpus.

Subfields: Ecology, Environmental Chemistry, Oceanography

View Paper: https://www.semanticscholar.org/paper/dce4fed9ce56d937cf7770c9a1a95542ecbcce19

How researchers see this paper

The researchers whose trusted networks rank it highest, strongest advocate first. Every researcher gets a different order.

In Ecology

1. M. Mulholland
2. D. Hutchins
3. P. Bernhardt
4. C. Gobler
5. D. Wall
6. David L. Kirchman
7. T. Davis
8. J. Aislabie

In Environmental Chemistry

1. M. Mulholland
2. C. Gobler
3. D. Hutchins
4. P. Bernhardt
5. Dianna L. Berry
6. David L. Kirchman
7. T. Davis
8. Feixue Fu

In Oceanography

1. M. Mulholland
2. D. Hutchins
3. C. Gobler
4. P. Bernhardt
5. Dianna L. Berry
6. T. Davis
7. David L. Kirchman
8. D. Wall

Citations in the corpus

• A bacterium that inhibits the growth of Pfiesteria piscicida and other dinoflagellates (2004, background): “piscicida have also been documented (Coyne et al., 2001; Humphries, 2002).”
• REVIEW: The detection of aquatic animal species using environmental DNA – a review of eDNA as a survey tool in ecology (2014, methodology): “(2014) Mesocosm 250 mL 1 2 lm isopore membrane filter Filter stored at 20 °C Modified DNA extraction procedure (Coyne et al. 2001) Collins et al.”
• Co-variation in soil biodiversity and biogeochemistry in northern and southern Victoria Land, Antarctica (2006, methodology): “Nucleic acids were extracted from soil using a modified cetyltrimethyl-ammonium bromide-polyvinylpyrrolidoneβmercaptoethanol (CTAB) extraction protocol (Coyne et al. 2001) designed for maximum recovery of nucleic acids from low biomass silica-rich soils.”
• Understanding Causes and Impacts of the Dinoflagellate, Cochlodinium polykrikoides, Blooms in the Chesapeake Bay (2009, methodology): “Nucleic acids were extracted as in Coyne et al. (2001). The hypervariable D1–D3 region of the ribosomal DNA (rDNA) was amplified by polymerase chain reaction (PCR) using forward primer “D1RF” (5′ACCCGCTGAATTTAAGCATA-3′) and reverse primer “D3Car” (5′-ACGAACGATTTGCACGTCAG-3′; Mikulski et al.”
• Effects of nitrogenous compounds and phosphorus on the growth of toxic and non-toxic strains of Microcystis during cyanobacterial blooms (2010, methodology): “Total cellular nucleic acids were extracted from field and experimental samples using methods described in Coyne et al. (2001).”
• Characterization, dynamics, and ecological impacts of harmful Cochlodinium polykrikoides blooms on eastern Long Island, NY, USA (2008, unknown): “The resuspended cells were frozen at � 80 8C for at least 1 h. Nucleic acids were extracted as in Coyne et al. (2001) .”
• The use of quantitative polymerase chain reaction for the detection and enumeration of the harmful alga Aureococcus anophagefferens in environmental samples along the United States East Coast (2003, methodology): “Molecular techniques allow for the detection of harmful algal species at prebloom concentrations, as was demonstrated for Pfiesteria piscicida using PCR-fluorescent fragment detection (Coyne et al. 2001) and qPCR (Bowers et al. 2000).”