Distribution of miRNA expression across human tissues

By N. Ludwig, P. Leidinger, K. Becker, Christina Backes, Tobias Fehlmann, C. Pallasch, Stefanie Rheinheimer, B. Meder, C. Stähler, E. Meese and 1 more. Nucleic Acids Research, 2016

920 citations recorded by the source corpus.

We present a human miRNA tissue atlas by determining the abundance of 1997 miRNAs in 61 tissue biopsies of different organs from two individuals collected post-mortem. One thousand three hundred sixty-four miRNAs were discovered in at least one tissue, 143 were present in each tissue. To define the distribution of miRNAs, we utilized a tissue specificity index (TSI). The majority of miRNAs (82.9%) fell in a middle TSI range i.e. were neither specific for single tissues (TSI > 0.85) nor housekeeping miRNAs (TSI < 0.5). Nonetheless, we observed many different miRNAs and miRNA families that were predominantly expressed in certain tissues. Clustering of miRNA abundances revealed that tissues like several areas of the brain clustered together. Considering -3p and -5p mature forms we observed miR-150 with different tissue specificity. Analysis of additional lung and prostate biopsies indicated that inter-organism variability was significantly lower than inter-organ variability. Tissue-specific differences between the miRNA patterns appeared not to be significantly altered by storage as shown for heart and lung tissue. MiRNAs TSI values of human tissues were significantly (P = 10−8) correlated with those of rats; miRNAs that were highly abundant in certain human tissues were likewise abundant in according rat tissues. We implemented a web-based repository enabling scientists to access and browse the data (https://ccb-web.cs.uni-saarland.de/tissueatlas).

Subfields: Cancer Research, Molecular Biology

View Paper: https://www.semanticscholar.org/paper/dc9823564677f04a2e1f18bb6ed9f2c7fef80a0e

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In Cancer Research

  1. Ruben Garcia-Martin
  2. R. Kahn
  3. R. G. Ludwig
  4. M. Mori
  5. B. Brandao

In Molecular Biology

  1. Ruben Garcia-Martin
  2. R. Kahn
  3. R. G. Ludwig
  4. M. Mori
  5. B. Brandao

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