Green synthesis of carbon dots from Ocimum sanctum for effective fluorescent sensing of Pb2+ ions and live cell imaging

By Amit Kumar, A. R. Chowdhuri, Dipranjan Laha, Triveni Kumar Mahto, P. Karmakar, S. Sahu. Sensors and actuators. B, Chemical, 2017

360 citations recorded by the source corpus.

Abstract A simple, rapid and cost-effective approach is developed to synthesize fluorescent carbon dots (CDs) using the leaves of Ocimum sanctum as a carbon source for the first time. The as-synthesized CDs possess high stability in aqueous solution and exhibit strong fluorescence with quantum yield of 9.3%. We have explored the use of such CDs as a fluorescent sensor for Pb2+ ions detection, which is based on Pb2+ ions induced fluorescence quenching of CDs. More significantly the resultant CDs has excellent selectivity and sensitivity towards Pb2+ ions with a limit of detection (LOD) 0.59 nM and linear detection range of 0.01–1.0 μM. The practical use of synthesized CDs for detection of Pb2+ ions is demonstrated in triple negative breast cancer cells (MDA-MB 468 cells) and real water samples successfully. Moreover, the CDs are also possessing low cytotoxicity to exhibit excellent fluorescent probe for multicolour cellular imaging.

Subfields: Materials Chemistry, Molecular Biology, Spectroscopy

View Paper: https://www.semanticscholar.org/paper/dc609dc10dcc00186d9b6cc2b8545b0a17813a46