Nucleic Acid Detection Using CRISPR/Cas Bio-sensing Technologies.
By R. Aman, Ahmed Mahas, Magdy M. Mahfouz. ACS Synthetic Biology, 2020
327 citations recorded by the source corpus.
For infectious diseases, rapid, accurate identification of the pathogen is critical for effective management and treatment, but diagnosis remains challenging, particularly in resource-limited areas. Methods that accurately detect pathogen nucleic acids can provide robust, accurate, rapid, and ultra-sensitive technologies for point-of-care diagnosis of pathogens, and thus yield information that is invaluable for disease management and treatment. Several technologies, mostly PCR-based, have been employed for pathogen detection; however, these require expensive reagents and equipment, and skilled personnel. CRISPR/Cas systems have been used for genome editing, based on their ability to accurately recognize and cleave specific DNA and RNA sequences. Moreover, following recognition of the target sequence, certain CRISPR/Cas systems including orthologues of Cas13, Cas12a, and Cas14 exhibit collateral non-specific catalytic activities that can be employed for nucleic acid detection, for example by degradation of a labeled nucleic acid to produce a fluorescent signal. CRISPR/Cas systems are amenable to multiplexing, thereby enabling a single diagnostic test to identify multiple targets up to attomolar (10-18 mol/L) concentrations of target molecules. Developing devices that couple CRISPR/Cas with lateral flow systems may allow cheap, accurate, highly sensitive, in-field deployable diagnostics. These sensors have myriad applications, from human health to agriculture. In this review, we discuss the recent advances in the field of CRISPR-based biosensing technologies and highlight insights of their potential use in a myriad of applications.
Subfields: Molecular Biology, Plant Science, Public Health, Environmental and Occupational Health
View Paper: https://www.semanticscholar.org/paper/dc50fb155689089b4132ee59dd7daef465b32ed3
Citations in the corpus
- Point-of-care CRISPR/Cas nucleic acid detection: Recent advances, challenges and opportunities (2020, unknown): “In 2020 Aman et al. covered recent advances in the field of CRISPR-based biosensing technologies and highlighted insights into their potential use in multiple applications (Aman et al., 2020).”