Preparation of chitooligosaccharides with degree of polymerization higher than 6 by acid or enzymatic degradation of chitosan

By J. Cabrera, P. Cutsem. 2005

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Modification of Chitosan for the Generation of Functional Derivatives (2019, background): “Overall, chemical processes include chitosanolysis with HCl [56], HNO2 [57], H2O2 [58], and potassium persulfate [59].”
A future perspective in crop protection chitosan and its oligosaccharides (2014, background): “Chemical hydrolysis is performed at high temperatures under highly acidic conditions, mainly using HCl or HNO2 and produces a large amount of glucosamine [103].”
The effect of size and acetylation degree of chitosan derivatives on tobacco plant protection against Phytophthora parasitica nicotianae (2007, unknown): “We decided to take advantage of either, the availability and price of a commercial preparation, which contains such enzymatic activity, and the possibility of preparing hydrolysates enriched in chitosan fragments with DP higher than six, taking into account recent results with this enzymatic prepara”
Chitosan: An Overview of Its Properties and Applications (2021, methodology): “By using a controlled precipitation method with methanol, it has been possible to obtain chitooligosaccharides with DPs up to 16 with few low molecular weight oligomers with a good yield [38].”