Gold nanoparticle sensor for the visual detection of pork adulteration in meatball formulation

By Md. Eaqub Ali, Uda Hashim, S. Mustafa, Y. C. Man, K. Islam. 2012

191 citations recorded by the source corpus.

We visually identify pork adulteration in beef and chicken meatball preparations using 20nm gold nanoparticles (GNPs) as colorimetric sensors. Meatball is a popular food in certain Asian and European countries. Verification of pork adulteration in meatball is necessary to meet the Halal and Kosher food standards. Twentynm GNPs change color from pinkish-red to gray-purple, and their absorption peak at 525nm is red-shifted by 30-50nm in 3mM phosphate buffer saline (PBS). Adsorption of single-stranded DNA protects the particles against salt-induced aggregation. Mixing and annealing of a 25-nucleotide (nt) single-stranded (ss) DNA probe with denatured DNA of different meatballs differentiated well between perfectly matched and mismatch hybridization at a critical annealing temperature. The probes become available in nonpork DNA containing vials due to mismatches and interact with GNPs to protect them from salt-induced aggregation. Whereas, all the pork containing vials, either in pure and mixed forms, consumed the probes totally by perfect hybridization and turned into grey, indicating aggregation. This is clearly reflected by a well-defined red-shift of the absorption peak and significantly increased absorbance in 550-800nm regimes. This label-free low-cost assay should find applications in food analysis, genetic screening, and homology studies.

Subfields: Electronic, Optical and Magnetic Materials, Food Science, Molecular Biology

View Paper: https://www.semanticscholar.org/paper/d9122926cd8549173c37bdc716c1bb1b3328957a

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S. Mustafa
M. E. Ali
U. Hashim
Y. Man
Md. Eaqub Ali

Citations in the corpus

Swine-Specific PCR-RFLP Assay Targeting Mitochondrial Cytochrome B Gene for Semiquantitative Detection of Pork in Commercial Meat Products (2012, background): “Nanotechnology-based colorimetric detection of specific sequences is seemingly simple and interesting, but lacks the required sensitivity and suffers from the interference of single-stranded nucleic acid species (Ali et al. 2011a, 2012).”