Investigations into the antibacterial behavior of copper nanoparticles against Escherichia coli

By M. Raffi, Saba Mehrwan, T. Bhatti, Javed Akhter, A. Hameed, W. Yawar, M. Masood ul Hasan. Annals of Microbiology, 2010

646 citations recorded by the source corpus.

Zerovalent copper nanoparticles (Cu0) of 12 nm size were synthesized using an inert gas condensation method in which bulk copper metal was evaporated into an inert environment of argon with subsequent cooling for nucleation and growth of nanoparticles. Crystalline structure, morphology and estimation of size of nanoparticles were carried out by X-ray diffraction and transmission electron microscopy. The antibacterial activity of these nanoparticles against the Gram-negative bacterium Escherichia coli was assessed in liquid as well as solid growth media. It was observed from scanning electron microscopic analysis that the interaction of copper nanoparticles with E. coli resulted in the formation of cavities/pits in the bacterial cell wall. The antibacterial property of copper nanoparticles was attributed mainly to adhesion with bacteria because of their opposite electrical charges, resulting in a reduction reaction at the bacterial cell wall. Nanoparticles with a larger surface-to-volume ratio provide more efficient means for antibacterial activity.

Subfields: Biomedical Engineering, Electronic, Optical and Magnetic Materials, Materials Chemistry

View Paper: https://www.semanticscholar.org/paper/d84c9cd6d9ce3991f09ae05b97b21024b5b8008b

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In Biomedical Engineering

1. T. Basu
2. A. Chatterjee
3. Ruchira Chakraborty

In Materials Chemistry

1. T. Basu
2. A. Chatterjee
3. Ruchira Chakraborty

Citations in the corpus

• Investigation of the antibacterial properties of nanocopper against Xanthomonas axonopodis pv. punicae, the incitant of pomegranate bacterial blight (2011, background): “…which have a larger surface-to-volume ratio, are more efficient for antibacterial activity against Gram negative bacteria, as previously reported for Escherichia coli (Raffi et al. 2010), Xanthomonas axonopodis pv. phaseoli, and Xanthomonas oryzae pv. oryzae (Mondal 2009; Mondal et al. 2010).”
• Mechanism of antibacterial activity of copper nanoparticles (2014, background): “Raffi et al [4] and Ruparelia et al [5] suggested that Cu ions originating from the NPs may interact with phosphorus and sulfur-containing biomolecules such as DNA and protein to distort their structures and thus disrupt biochemical processes.”