Microbial Interkingdom Interactions in Roots Promote Arabidopsis Survival

By Paloma Durán, Thorsten Thiergart, Ruben Garrido-Oter, Matthew T. Agler, E. Kemen, Paul Schulze-Lefert, S. Hacquard. bioRxiv, 2018

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Roots of healthy plants are inhabited by soil-derived bacteria, fungi, and oomycetes that have evolved independently in distinct kingdoms of life. How these microorganisms interact and to what extent those interactions affect plant health are poorly understood. We examined root-associated microbial communities from three Arabidopsis thaliana populations and detected mostly negative correlations between bacteria and filamentous microbial eukaryotes. We established microbial culture collections for reconstitution experiments using germ-free A. thaliana. In plants inoculated with mono- or multi-kingdom synthetic microbial consortia, we observed a profound impact of the bacterial root microbiota on fungal and oomycetal community structure and diversity. We demonstrate that the bacterial microbiota is essential for plant survival and protection against root-derived filamentous eukaryotes. Deconvolution of 2,862 binary bacterial-fungal interactions ex situ, combined with community perturbation experiments in planta, indicate that biocontrol activity of bacterial root commensals is a redundant trait that maintains microbial interkingdom balance for plant health.

Subfields: Plant Science

View Paper: https://www.semanticscholar.org/paper/d7f5c1b313453e41cb03106a317bba88b707961c

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Citations in the corpus

Network analysis methods for studying microbial communities: A mini review (2021, methodology): “…Linear system using log ratios between pairs of compositions with l1-norm shrinkage method [107] Interaction studies of ecological systems like ranging from plant [110,111], soil [112] Able to reproduce and identify the changes between infants gut micro-biome and older children and adults [56]”
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Agricultural intensification reduces microbial network complexity and the abundance of keystone taxa in roots (2018, background): “It has also been observed that the impact of abiotic factors and host genotypes on the plant microbiome is facilitated via keystone taxa [51], and the root microbial network complexity is linked to plant survival [52].”