Overexpression of Cytosolic Ascorbate Peroxidase in Tomato Confers Tolerance to Chilling and Salt Stress

By Yueju Wang, Michael Wisniewski, R. Meilan, Minggang Cui, R. Webb, L. Fuchigami. 2005

161 citations recorded by the source corpus.

Ascorbate peroxidase (APX) plays an important role in the metabolism of hydrogen peroxide in higher plants, affording them protection against oxidative stress. We studied the effect of overexpressing a cytosolic ascorbate peroxidase (cAPX) gene—derived from pea (Pisum sativum L.)—in transgenic tomato plants (Lycopersicon esculentum L.). Transformants were selected in vitro using kanamycin resistance and confi rmed by polymerase chain reaction (PCR) and northern analyses. An APX native-gel assay indicated that, in the absence of stress, APX activity in transgenic plants was several times greater than that measured in wild-type (WT) plants. Several independently transformed lines were propagated and evaluated for resistance to chilling and salt stress. After placing seeds at 9 °C for 5 weeks, percent germination was greater for seeds obtained from transgenic lines (26% to 37%) compared to the WT (3%). Plants from transgenic lines also had lower electrolyte leakage (20% to 23%) than WT (44%) after exposure to 4 °C. Visual assessment of transgenic and WT lines exposed to salinity stress (200 or 250 mM) confi rmed that overexpression of APX minimized leaf damage. Moreover, APX activity was nearly 25- and 10-fold higher in the leaves of transgenic plants in response to chilling and salt stresses, respectively. Our results substantiate that increased levels of APX activity brought about by overexpression of a cytosolic APX gene may play an important role in ameliorating oxidative injury induced by chilling and salt stress.

Subfields: Materials Chemistry, Plant Science

View Paper: https://www.semanticscholar.org/paper/d5bd68ad8d4fa0920be3ae62e638cefef4467fd7

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1. Prachi Pandey
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Citations in the corpus

• Redox homeostasis via gene families of ascorbate-glutathione pathway (2015, background): “Transgenic L. esculentum plants over-expressing cytosolic APX exhibited improved tolerance to chilling, salinity, heat and UVB stress (Wang et al., 2005, 2006).”
• Recent Developments in Enzymatic Antioxidant Defence Mechanism in Plants with Special Reference to Abiotic Stress (2021, background): “APX has been reported from different sub-cellular locations in the plants, such as in the cytosol [141], mitochondria [142]”
• Reactive oxygen species and antioxidant machinery in abiotic stress tolerance in crop plants. (2010, unknown): “stresses, increase in APX activity [280,281]”
• Reactive Oxygen Species, Oxidative Damage, and Antioxidative Defense Mechanism in Plants under Stressful Conditions (2012, unknown): “) ameliorated oxidative injury induced by chilling and salt stress [209].”
• Changes in antioxidant enzymes activity and plant performance by salinity stress and zinc application in soybean ( Glycine max L.) (2012, unknown): “in ROS formation (Wang et al., 2005).”