Methods for the detection of beef and pork in foods using real-time polymerase chain reaction

By I. Laube, A. Spiegelberg, A. Butschke, J. Zagon, M. Schauzu, L. Kroh, H. Broll. 2003

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Multiplex PCR in Species Authentication: Probability and Prospects—A Review (2014, background): “…Dooley et al. (2004) Haddock Transferrin – Hird et al. (2004) Cattle Growth hormone 0.02 Brodmann and Moor (2003) Cattle and pork Phosphodiesterase, ryanodine gene – Laube et al. (2003) Molecular Beacon Chemistry Pork cytb 0.0001 Hazim et al. (2012) SYBR Green chemistry Tuna species…”
Multiplex real-time PCR for the detection and quantification of DNA from beef, pork, horse and sheep (2008, methodology): “PCR techniques proved their applicability for the analysis of mixed processed food already in the past [2–17].”
Comparison of real-time PCR and ELISA-based methods for the detection of beef and pork in processed meat products (2017, methodology): “On the 237 other hand, Laube et al. (2003) showed slightly greater sensitivity for beef detection at 0.1% w/w 238 in a beef/pork mixture tested with real-time PCR. 239 3.2.”