Moving environmental DNA methods from concept to practice for monitoring aquatic macroorganisms
By Caren S. Goldberg, Katherine M. Strickler, David S. Pilliod. 2015
276 citations recorded by the source corpus.
The discovery that macroorganisms can be detected from their environmental DNA (eDNA) in aquatic systems has immense potential for the conservation of biological diversity. This special issue contains 11 papers that review and advance the field of eDNA detection of vertebrates and other macroorganisms, including studies of eDNA production, transport, and degradation; sample collection and processing to maximize detection rates; and applications of eDNA for conservation using citizen scientists. This body of work is an important contribution to the ongoing efforts to take eDNA detection of macroorganisms from technical breakthrough to established, reliable method that can be used in survey, monitoring, and research applications worldwide. While the rapid advances in this field are remarkable, important challenges remain, including consensus on best practices for collection and analysis, understanding of eDNA diffusion and transport, and avoidance of inhibition in sample collection and processing. Nonetheless, as demonstrated in this special issue, eDNA techniques for research and monitoring are beginning to realize their potential for contributing to the conservation of biodiversity globally.
Subfields: Ecology, Molecular Biology
View Paper: https://www.semanticscholar.org/paper/d4b89dd9e53a1c3bdeca4398a4d6cb1f47c49669
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In Ecology
Citations in the corpus
- DNA-based methods in paleolimnology: new opportunities for investigating long-term dynamics of lacustrine biodiversity (2017, methodology): “Molecular techniques were employed successfully to detect other invasive organisms, including vertebrates, invertebrates, plants and pathogens (Jerde et al. 2011; Mahon et al. 2013; Goldberg et al. 2015), by analysis of water samples; consequently some public agencies are now implementing eDNA analy”
- Meta‐analysis supports further refinement of eDNA for monitoring aquatic species‐specific abundance in nature (2019, background): “Multiple factors can affect the quantity of eDNA detected, which is a function of production, deg‐ radation, and transport of eDNA particles (Goldberg et al., 2015).”