Label-Free Probing of the Binding State of DNA by Time-Domain Terahertz Sensing

By M. Brucherseifer, Michael Nagel, P. H. Bolivar, Heinrich Kurz, A. Bosserhoff, R. Büttner. 2000

424 citations recorded by the source corpus.

We present a promising approach for the label-free characterization of genetic material. Time-resolved terahertz (THz) transmission analysis of polynucleotides demonstrate a strong dependence of the complex refractive index on the binding state (hybridized/denatured) of deoxyribonucleic acid (DNA) molecules. By monitoring THz transients, one can thus infer the binding state of oligo- and polynucleotides, and hence identify polynucleotides by detecting the binding of unknown polynucleotide DNA sequences to known probe molecules. A broadband experimental demonstration in a free-space configuration, as well as a discussion of the potential application for next generation gene chips is presented.

Subfields: Atomic and Molecular Physics, and Optics, Electrical and Electronic Engineering

View Paper: https://www.semanticscholar.org/paper/d469bccde6c1fc2804a58a8d6634ae1aae5fce56

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In Atomic and Molecular Physics, and Optics

In Electrical and Electronic Engineering

Citations in the corpus

Metamaterials Application in Sensing (2012, methodology): “This method is reported to have increased sensitivity to the binding state of DNA samples by ~10(3) times over conventional free-space time-domain spectroscopy [78].”
Terahertz Dielectric Sensitivity to Biomolecular Structure and Function (2008, background): “THz sensitivity to biomolecular binding has been demonstrated [ 90 ], [92] and it has been demonstrated in solution [56].”
Materials for terahertz science and technology (2002, background): “THz spectroscopy has shown the capability of differentiating between single- and double-stranded DNA owing to associated changes in refractive inde”