Genome-wide recessive genetic screening in mammalian cells with a lentiviral CRISPR-guide RNA library

By Hiroko Koike-Yusa, Yilong Li, E-Pien Tan, M. D. C. Velasco-Herrera, K. Yusa. Nature Biotechnology, 2013

1,011 citations recorded by the source corpus.

Subfields: Genetics, Molecular Biology

View Paper: https://www.semanticscholar.org/paper/d329adf6a5d0759b2c2cf24d72982cebe5fbd7e0

How researchers see this paper

The researchers whose trusted networks rank it highest, strongest advocate first. Every researcher gets a different order.

In Genetics

1. Lei S. Qi
2. Daniel G. Anderson
3. Aviv Regev
4. Feng Zhang
5. Charles Boone
6. Robert Langer
7. G. Feng
8. Brenda J. Andrews

In Molecular Biology

1. Lei S. Qi
2. Feng Zhang
3. Jonathan S. Weissman
4. Luke A. Gilbert
5. Daniel G. Anderson
6. Aviv Regev
7. Jennifer A. Doudna
8. Robert Langer

Citations in the corpus

• Evaluation and Design of Genome-Wide CRISPR/SpCas9 Knockout Screens (2017, methodology): “…et al. 2015; Steinhart et al. 2017), four suspension cell lines using the Sabatini library (Wang et al. 2015), and five suspension plus one adherent line screened with the Yusa library (Koike-Yusa et al. 2014; Tzelepis et al. 2016), for a total of 17 wholegenome screens (Figure 1A and Table S1).”
• CRISPR-Cas9 Knockin Mice for Genome Editing and Cancer Modeling (2014, background): “Given recent successes using Cas9 and large-scale sgRNA libraries for genetic screening (Findlay et al., 2014; Koike-Yusa et al., 2014; Sanjana et al., 2014; Shalem et al., 2014; Wang et al., 2014; Zhou et al., 2014), the Cas9 mouse may be directly combined with genome-wide or targeted sgRNA…”
• Evaluation of off-target and on-target scoring algorithms and integration into the guide RNA selection tool CRISPOR (2016, methodology): “[54] but were used as processed by Xu et al.”
• Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9 (2016, neutral): “The distribution of Rule Set 1 scores for the previously-published GeCKO 6,15 and Koike-Yusa et al. 8 libraries resembles the null distribution, as these were not designed with on-target criteria ( Fig.”
• A Genome-Wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes (2016, neutral): “A uthor M anuscript A uthor M anuscript A uthor M anuscript A uthor M anuscript Several studies have developed CRISPR/Cas9-based genome-wide genetic screens for mammalian cells (Koike-Yusa et al., 2014; Shalem et al., 2014; Wang et al., 2014).”