Comparison of Fatty Acid Methyl Ester (FAME) Methods for Characterizing Microbial Communities

By M. Schutter, R. Dick. 2000

424 citations recorded by the source corpus.

Subfields: Agronomy and Crop Science, Environmental Chemistry, Soil Science

View Paper: https://www.semanticscholar.org/paper/d07b5205acc423dd31c244d9965dfc609f35d7b9

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In Agronomy and Crop Science

In Environmental Chemistry

In Soil Science

Citations in the corpus

Disentangling the impact of AM fungi versus roots on soil structure and water transport (2008, methodology): “Fatty acids were extracted from soil samples using the ester-linked methods described in detail by Schutter and Dick (2000) ; 2 g of soil mixed with 15 ml of 0.2 M KOH in methanol and 30 μl internal standard added to culture tubes.”
Change in soil properties and the soil microbial community following land spreading of olive mill wastewater affects olive trees key physiological parameters and the abundance of arbuscular mycorrhizal fungi (2008, methodology): “Lipids were extracted from the soil samples (3 g) as well as the root samples (30mg) using the EL-FAME method (Schutter and Dick, 2000).”
Increased N availability in grassland soils modifies their microbial communities and decreases the abundance of arbuscular mycorrhizal fungi (2006, methodology): “, 2000) and has been used by several investigators as an alternative to the strong hydrolysis conditions in the ‘MIDI-FAME’ approach while maintaining a straight-forward extraction procedure applicable to processing large numbers of samples (Ritchie et al., 2000; Schutter and Dick, 2000; Nazih et al”
Soil microbial community structure in European forests in relation to forest type and atmospheric nitrogen deposition (2011, methodology): “Community FAME profiles were determined using the ester linked (EL) FAME method (Schutter and Dick 2000).”
Pyrosequencing analysis for characterization of soil bacterial populations as affected by an integrated livestock-cotton production system (2010, methodology): “Schutter and Dick (2000) reported that some FAME indicators for G bacteria (i.e., i17:0 3OH) were extracted in significantly greater quantities (P 0.05) with the MIDI protocol compared to an esterlinked (EL) method, which in theory can extract only ester-linked (not free) fatty acids from soil.”
Effects of agronomic application of olive mill wastewater in a field of olive trees on carbohydrate profiles, chlorophyll a fluorescence and mineral nutrient content (2011, methodology): “Lipids were extracted from the soil samples (3 g) as well as the root samples (30mg) using the EL-FAME method (Schutter and Dick, 2000).”
Trophic relations in a stream food web: importance of fatty acids for macroinvertebrate consumers (2007, methodology): “Correlations between distances in the reduced NMS space and distances in the original space were used to quantify the proportion of total variation explained by NMS, an analysis similar to that used with FA profiles of soil microbial communities (Shutter and Dick 2000, Butler et al. 2003).”
Soil microbial communities under different soybean cropping systems: characterization of microbial population dynamics, soil microbial activity, microbial biomass, and fatty acid profiles. (2009, background): “Some studies have confirmed that whole-community FAME profiles constitute reproducible signature useful to characterize soil microbial communities (Schutter and Dick, 2000; Drenovsky et al., 2004).”
Dynamics of bacterial communities in relation to soil aggregate formation during the decomposition of 13C-labelled rice straw (2012, methodology): “The analysis of microbial community structure using FAME profiling to distinguish microbial communities from different soils is now well established and gives results that are similar to DNAbased methods (Ritchie et al., 2000; Schutter and Dick, 2000).”
Effect of land management and Prosopis juliflora (Sw.) DC trees on soil microbial community and enzymatic activities in intensive silvopastoral systems of Colombia (2012, methodology): “Microbial community analyses Fatty acids were extracted from soil samples using the total ster-linked fatty acid methyl ester (EL-FAME) method as described y Schutter and Dick (2000).”
Microbial community composition and enzyme activities in a sandy loam soil after fumigation with methyl bromide or alternative biocides (2006, methodology): “[MIDI], Newark, Delaware, USA) provides a fast, simple, cost effective, and reproducible method (Cavigelli et al., 1995; Ibekwe and Kennedy, 1999; Schutter and Dick, 2001; Acosta-Marti´nez et al., 2004a,b).”