High-level production of uniformly 15N-and 13C-enriched fusion proteins in Escherichia coli

By M. Jansson, Yu-Chin Li, L. Jendeberg, S. Anderson, Gaetano T. Montelione, B. Nilsson. 1996

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The High-Throughput Protein Sample Production Platform of the Northeast Structural Genomics Consortium (2010, methodology): “5 ml of MJ9 minimal media (Jansson et al., 1996) for overnight growth.”
A microscale protein NMR sample screening pipeline (2009, methodology): “The entire volumes of each overnight culture is then used to inoculate a 67 ml fermentation in a 100 ml tube (Midi Scale Fermentation) containing MJ9 supplemented with uniformly U-15NH4-salts (1–2 g/l) and a mixture of 100% U-13C-glucose (5”
Preparation of protein samples for NMR structure, function, and small-molecule screening studies. (2011, methodology): “Following the allotted time, the BioRobot transfers 10 μL of each well into the corresponding well of a fresh 96-well S-block containing 0.5 mL (per well) of MJ9 minimal media (Jansson et al., 1996).”
Automated protein fold determination using a minimal NMR constraint strategy (2003, methodology): “coli RV308 was first grown in MJ/H2O minimal medium (Jansson et al. 1996) at 37°C to OD ≈1.”
A method for efficient isotopic labeling of recombinant proteins (2001, background): “Minimal media supplements such as trace metal mixtures, vitamin cocktails as well as commercially available algal and microbial hydrolysates have shown enhancements in growth and expression (Reilly and Fairbrother, 1994; Jansson et al., 1996; Kainosho, 1997; Cai et al., 1998).”