Transferrin Receptor Is a Specific Ferroptosis Marker
By Huizhong Feng, K. Schorpp, Jenny Jin, Carrie E. Yozwiak, B. Hoffstrom, Aubrianna Decker, Presha Rajbhandari, Michael E. Stokes, H. G. Bender, J. Csuka and 6 more. Cell Reports, 2020
755 citations recorded by the source corpus.
SUMMARY Ferroptosis is a type of regulated cell death driven by the iron-dependent accumulation of oxidized polyunsaturated fatty acid-containing phospholipids. There is no reliable way to selectively stain ferroptotic cells in tissue sections to characterize the extent of ferroptosis in animal models or patient samples. We address this gap by immunizing mice with membranes from lymphoma cells treated with the ferroptosis inducer piperazine erastin and screening ~4,750 of the resulting monoclonal antibodies generated for their ability to selectively detect cells undergoing ferroptosis. We find that one antibody, 3F3 ferroptotic membrane antibody (3F3-FMA), is effective as a selective ferroptosis-staining reagent. The antigen of 3F3-FMA is identified as the human transferrin receptor 1 protein (TfR1). We validate this finding with several additional anti-TfR1 antibodies and compare them to other potential ferroptosis-detecting reagents. We find that anti-TfR1 and anti-malondialdehyde adduct antibodies are effective at staining ferroptotic tumor cells in multiple cell culture and tissue contexts.
Subfields: Cancer Research, Molecular Biology, Pulmonary and Respiratory Medicine
View Paper: https://www.semanticscholar.org/paper/cf58be0eb87dd9929b7d71386185c30c81a5b7c6
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Citations in the corpus
- The cell biology of ferroptosis (2024, neutral): “However, since lipid peroxidation and 4-hydroxynonenal accumulation are not entirely specific to ferroptosis, identifying additional markers of this process that can be used to monitor ferroptosis in vivo remains a priority 187,191,201,209 .”
- Regulated cell death (RCD) in cancer: key pathways and targeted therapies (2022, background): “The upregulated TfR expression on the surface of various tumor cells not only provides a natural advantage for more effective iron-mediated iron endocytosis, but also provides a target for therapeutic drug delivery.(483) PL is loaded as a ferroptosis inducer in an iron-containing MOF coated with a d”
- Lipid Peroxidation and Iron Metabolism: Two Corner Stones in the Homeostasis Control of Ferroptosis (2022, background): “Interestingly, TfR1 was also identified and validated as a ferroptosis marker among other proposed biomarkers [82].”
- Ferroptosis turns 10: Emerging mechanisms, physiological functions, and therapeutic applications. (2022, positive): “My lab recently reported that increased abundance and plasma localization of the Tf receptor TfR1 is a marker for ferroptosis, which can be detected with the newly identified 3F3-FMA antibody, as well as with additional anti-TfR1 antibodies (Feng et al., 2020).”