Development and characterization of an ELISA assay in PDMS microfluidic channels

By E. Eteshola, D. Leckband. 2001

286 citations recorded by the source corpus.

Abstract This report describes the first preliminary evaluation of a heterogeneous sandwich enzyme-linked immunoassay in a PDMS microfluidic device. The PDMS devices were fabricated using replica molding against a patterned photoresist generated by photolithographic techniques. With this experimental setup, the microfluidic sensor chip was successfully used to quantify a model analyte (sheep IgM) with sensitivity down to 17 nM. The conventional blocking cocktail used in nearly all polystyrene microtiter plate-based ELISA assays failed to block the nonspecific adsorption of the analyte and secondary antibody. This report describes the successful use of surface modification chemistries and a modified blocking cocktail to reduce background due to nonspecific adsorption and to thereby increase the sensitivity of the assay. These protocols can be extended to the detection of a variety of analytes by immunoassay in PDMS microchannels.

Subfields: Biomedical Engineering

View Paper: https://www.semanticscholar.org/paper/cf249c2dff021f159155e8acd2532c18b6433752