Semi-automated data processing of hydrogen exchange mass spectra using HX-Express

By D. Weis, John R. Engen, Ignatius J Kass. 2006

266 citations recorded by the source corpus.

A Microsoft Excel utility, HX-Express, that significantly accelerates the analysis of hydrogen exchange mass spectrometry data is described. HX-Express generates deuterium uptake and peak width plots from peaks in mass spectral data. Data analysis is intentionally semi-automated, requiring that the user find the peaks to be analyzed. The peaks are entered in the form of x, y lists of m/z versus intensity or can be directly imported from Waters MassLynx software. Analysis of data with HX-Express provides the same results as manual data processing but is substantially faster. In addition to speed, HX-Express provides and preserves visual and numeric displays of the analysis process for quality control.

Subfields: Molecular Biology, Spectroscopy

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The researchers whose trusted networks rank it highest, strongest advocate first. Every researcher gets a different order.

In Molecular Biology

1. W. Sheffler
2. David Baker
3. Neil P. King
4. Lauren P. Carter
5. D. Veesler
6. A. Walls
7. M. Navarro
8. F. Sallusto

In Spectroscopy

1. D. Weis
2. Kelly K. Lee
3. David Baker
4. John R. Engen
5. Oliver F. Lange
6. Po-Ssu Huang
7. Raphael R. Eguchi

Citations in the corpus

• Elicitation of Potent Neutralizing Antibody Responses by Designed Protein Nanoparticle Vaccines for SARS-CoV-2 (2020, positive): “Deuterium uptake analysis was performed with HX-Express v2 (Guttman et al., 2013; Weis et al., 2006).”
• Induction of Potent Neutralizing Antibody Responses by a Designed Protein Nanoparticle Vaccine for Respiratory Syncytial Virus (2019, background): “(A) Raw spectra for certain peptides displayed spectral broadening andwere better fit by bimodal deconvolution (Guttman et al., 2013;Weis et al., 2006).”
• Analysis of Overlapped and Noisy Hydrogen/Deuterium Exchange Mass Spectra (2013, methodology): “In the last decade, many H/D analysis software packages have emerged, both open source and commercial; AutoHD [5], HX-Express [6], DEX [7], Hydra [8], HeXicon [9], Ex-MS [10], HD-Benchtop [11], Mass Analyzer [12], HD-examiner (Sierra Analytics), and DynamX (Waters), to name a few.”
• Elicitation of broadly protective immunity to influenza by multivalent hemagglutinin nanoparticle vaccines (2020, unknown): “88,89 ).”
• HDX Workbench: Software for the Analysis of H/D Exchange MS Data (2012, unknown): “methods and tools [2–17].”