Postnatal development of kisspeptin neurons in mouse hypothalamus; sexual dimorphism and projections to gonadotropin-releasing hormone (GnRH) neurons
By J. Clarkson, A. Herbison. Endocrinology, 2006
823 citations recorded by the source corpus.
The neuropeptide kisspeptin has recently been implicated as having a critical role in the activation of the gonadotropin-releasing hormone (GnRH) neurons to bring about puberty. We examined here the postnatal development of kisspeptin neuronal populations and their projections to GnRH neurons in the mouse. Three populations of kisspeptin neurons located in the (1) anteroventral periventricular nucleus (AVPV) and the preoptic periventricular nucleus (PeN), (2) dorsomedial nucleus (DMN), and (3) arcuate nucleus (ARN) were identified using an antisera raised against mouse kisspeptin-10. A marked 10-fold (p<0.01), female-dominant, sex difference in the numbers of kisspeptin neurons existed in the AVPV/PeN but not elsewhere. Kisspeptin neurons in the AVPV/PeN of both sexes displayed a similar pattern of postnatal development with no cells detected at postnatal day 10 (P10) followed by increases from P25 to reach adult-levels by puberty onset (p<0.01; P31 females & P45 males). This pattern was not found in the DMN or ARN. Dual immunofluorescence experiments demonstrated close appositions between kisspeptin fibers and GnRH neuron cell bodies that were first apparent at P25 and increased across postnatal development in both sexes. These studies demonstrate kisspeptin peptide expression in the mouse hypothalamus and reveal the postnatal development of a sexually dimorphic continuum of kisspeptin neurons within the AVPV and PeN. This periventricular population of kisspeptin neurons reaches adult-like proportions at the time of puberty onset and is the likely source of the kisspeptin inputs to GnRH neurons.
Subfields: Molecular Biology, Reproductive Medicine
View Paper: https://www.semanticscholar.org/paper/cd59bbc782019e96bb4d65d23595f1c6833adf89
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In Reproductive Medicine
Citations in the corpus
- Kisspeptins and reproduction: physiological roles and regulatory mechanisms. (2012, background): “Also of interest, this study disclosed that, despite abundant expression of Kiss1 mRNA, the number of kisspeptin-IR cells in the AVPV is surprisingly low in the adult female rat (99), in contrast to previous reports in mice (66, 69).”
- Hypothalamic–pituitary–adrenal and hypothalamic–pituitary–gonadal axes: sex differences in regulation of stress responsivity (2017, background): “Moreover, Clarkson and Herbison (2006) detected a female bias in the levels of kisspeptin expression in the anteroventral and preoptic periventricular nucleus of the mouse hypothalamus (Clarkson & Herbison, 2006).”