How to do successful gene expression analysis using real-time PCR.

By Stefaan Derveaux, Jo Vandesompele, Jan Hellemans. Methods, 2010

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Comprehending crystalline β-carotene accumulation by comparing engineered cell models and the natural carotenoid-rich system of citrus (2011, neutral): “While sensitive and precise, one major drawback of the technique is the numerous critical steps required throughout the entire workflow that may influence the accuracy and reliability of results (Huggett et al., 2005; Derveaux et al., 2010; Huggett and Bustin, 2011).”
Advanced spectroscopic techniques for plant disease diagnostics. A review (2019, background): “PCR and, in particular, quantitative (q)PCR, are the work horses of plant pathology [13,14].”
Reference genes in real-time PCR (2013, methodology): “…in Germany (March 2004), continued further on in different countries as lead by Prof. Stephen Bustin, and the publication of MIQE Guidelines which goes for Minimum Information for Publication of Quantitative Real-Time PCR Experiments (Bustin et al. 2009; Bustin et al. 2010; Derveaux et al. 2010).”