Detection of a novel human coronavirus by real-time reverse-transcription polymerase chain reaction.

By V. Corman, I. Eckerle, T. Bleicker, A. Zaki, O. Landt, M. Eschbach-Bludau, S. V. Boheemen, R. Gopal, M. Ballhause, T. Bestebroer and 7 more. Euro surveillance : bulletin Europeen sur les maladies transmissibles = European communicable disease bulletin, 2012

606 citations recorded by the source corpus.

We present two real-time reverse-transcription polymerase chain reaction assays for a novel human coronavirus (CoV), targeting regions upstream of the E gene (upE) or within open reading frame (ORF)1b, respectively. Sensitivity for upE is 3.4 copies per reaction (95% confidence interval (CI): 2.5–6.9 copies) or 291 copies/mL of sample. No cross-reactivity was observed with coronaviruses OC43, NL63, 229E, SARS-CoV, nor with 92 clinical specimens containing common human respiratory viruses. We recommend using upE for screening and ORF1b for confirmation.

Subfields: Infectious Diseases

View Paper: https://www.semanticscholar.org/paper/cbe8379cb4175d8297ddde7982a4445f93f1c432

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In Infectious Diseases

1. Marion Koopmans
2. Vittoria Colizza
3. Christian Drosten
4. Burtram C. Fielding

Citations in the corpus

• Middle East respiratory syndrome coronavirus in dromedary camels: an outbreak investigation (2013, methodology): “17, 18 We used RNA dilutions isolated from a MERS-CoV isolate EMC stock as a standard control.”
• Epidemiological, demographic, and clinical characteristics of 47 cases of Middle East respiratory syndrome coronavirus disease from Saudi Arabia: a descriptive study (2013, methodology): “Clinical samples were screened at the Ministry of Health laboratory with real-time RT-PCR. 23, 24 Amplifi cation targeted both the upstream E protein (upE gene) and ORF1a for confi rmation, and these are standard assays used in Saudi Arabia for MERS-CoV testing.”
• Detection of 2019 novel coronavirus (2019-nCoV) by real-time RT-PCR (2020, methodology): “Respiratory samples were obtained during 2019 from patients hospitalised at Charité medical centre and tested by the NxTAG respiratory pathogen panel (Luminex, S ́Hertogenbosch, The Netherlands) or in cases of MERS-CoV by the MERS-CoV upE assay as published before [10].”
• On the Use of Human Mobility Proxies for Modeling Epidemics (2013, background): “[65,66], is significant for developing countries as well, given they”
• Genomic Characterization of a Newly Discovered Coronavirus Associated with Acute Respiratory Distress Syndrome in Humans (2012, neutral): “The first generation of assays for this purpose has recently been described (45) .”
• The Role of Severe Acute Respiratory Syndrome (SARS)-Coronavirus Accessory Proteins in Virus Pathogenesis (2012, background): “Then, very recently, in September 2012 another novel coronavirus was identified in two patients, of which one died [8].”