The cellular thermal shift assay for evaluating drug target interactions in cells

By R. Jafari, Helena Almqvist, Hanna Axelsson, M. Ignatushchenko, T. Lundbäck, P. Nordlund, D. M. Molina. Nature Protocols, 2014

1,335 citations recorded by the source corpus.

Subfields: Computational Theory and Mathematics, Molecular Biology, Radiology, Nuclear Medicine and Imaging

View Paper: https://www.semanticscholar.org/paper/cbe27dbf1e791daef3f288c9f2fe5d8921241683

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In Molecular Biology

1. R. Su
2. Jianjun Chen
3. Han-Ming Shen
4. Huilin Huang
5. Xiaolan Deng
6. Hengyou Weng
7. Chuan He

Citations in the corpus

• Targeting FTO suppresses cancer stem cell maintenance and immune evasion (2020, positive): “Cellular Thermal Shift Assay (CETSA): To determine whether CS1 or CS2 act as a direct ligand of FTO protein, CETSA was conducted with intact MONOMAC 6 as published previously (Jafari et al., 2014).”
• Target identification with quantitative activity based protein profiling (ABPP) (2017, methodology): “…methods including the previously mentioned AfBPP approach as well as novel protein stability focused approaches such as drug affinity responsive target stability [15,16] and the cellular thermal shift assay [98,99], which are based on biophysical principles of ligand-induced protein stabilization.”