Identification and Functional Characterization of Human Cd4+Cd25+ T Cells with Regulatory Properties Isolated from Peripheral Blood
By H. Jonuleit, E. Schmitt, M. Stassen, A. Tuettenberg, J. Knop, Alexander Enk. Journal of Experimental Medicine, 2001
1,408 citations recorded by the source corpus.
A subpopulation of peripheral human CD4+CD25+ T cells that expresses CD45RO, histocompatibility leukocyte antigen DR, and intracellular cytotoxic T lymphocyte–associated antigen (CTLA) 4 does not expand after stimulation and markedly suppresses the expansion of conventional T cells in a contact-dependent manner. After activation, CD4+CD25+ T cells express CTLA-4 on the surface detectable for several weeks. These cells show a G1/G0 cell cycle arrest and no production of interleukin (IL)-2, IL-4, or interferon (IFN)-γ on either protein or mRNA levels. The anergic state of CD4+CD25+ T cells is not reversible by the addition of anti-CD28, anti–CTLA-4, anti–transforming growth factor β, or anti–IL-10 antibody. However, the refractory state of CD4+CD25+ T cells was partially reversible by the addition of IL-2 or IL-4. These data demonstrate that human blood contains a resident T cell population with potent regulatory properties.
Subfields: Immunology
View Paper: https://www.semanticscholar.org/paper/cb12d54893c3746881b5a95f963798688831d9e1
How researchers see this paper
The researchers whose trusted networks rank it highest, strongest advocate first. Every researcher gets a different order.
In Immunology
- Shimon Sakaguchi
- K. Wing
- P. Krammer
- Angelika Schmidt
- Nina Oberle
- M. Miyara
- Tomoyuki Yamaguchi
- T. Nomura
Citations in the corpus
- Increase of regulatory T cells in the peripheral blood of cancer patients. (2003, background): “However, the precise role of CTLA-4 and TGF- for the functional properties of Tregs still remains controversial (6, 19, 20).”
- Decidual and peripheral blood CD4+CD25+ regulatory T cells in early pregnancy subjects and spontaneous abortion cases. (2004, background): “…Treg cells, CD4+CD25+ Treg cells play a role in the induction of tolerance (Sakaguchi et al., 1995; Taylor et al., 2001; Beacher-Allan et al., 2001; Jonuleit et al., 2001; Levings et al., 2001; Ng et al., 2001; Stephens et al., 2001; Cohen et al., 2002; Hoffmann et al., 2002; Word and Sakaguchi,…”