Bioprinting 3D microfibrous scaffolds for engineering endothelialized myocardium and heart-on-a-chip
By Yu Shrike Zhang, Andrea Arneri, S. Bersini, S. Shin, Kai Zhu, Zahra Goli-Malekabadi, Julio Aleman, C. Colosi, Fabio Busignani, Valeria Dell'Erba and 8 more. Biomaterials, 2016
824 citations recorded by the source corpus.
Engineering cardiac tissues and organ models remains a great challenge due to the hierarchical structure of the native myocardium. The need of integrating blood vessels brings additional complexity, limiting the available approaches that are suitable to produce integrated cardiovascular organoids. In this work we propose a novel hybrid strategy based on 3D bioprinting, to fabricate endothelialized myocardium. Enabled by the use of our composite bioink, endothelial cells directly bioprinted within microfibrous hydrogel scaffolds gradually migrated towards the peripheries of the microfibers to form a layer of confluent endothelium. Together with controlled anisotropy, this 3D endothelial bed was then seeded with cardiomyocytes to generate aligned myocardium capable of spontaneous and synchronous contraction. We further embedded the organoids into a specially designed microfluidic perfusion bioreactor to complete the endothelialized-myocardium-on-a-chip platform for cardiovascular toxicity evaluation. Finally, we demonstrated that such a technique could be translated to human cardiomyocytes derived from induced pluripotent stem cells to construct endothelialized human myocardium. We believe that our method for generation of endothelialized organoids fabricated through an innovative 3D bioprinting technology may find widespread applications in regenerative medicine, drug screening, and potentially disease modeling.
Subfields: Biomedical Engineering, Molecular Biology, Surgery
View Paper: https://www.semanticscholar.org/paper/ca8b0e0943b926f8735233d8b32bd35e4544841b
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Citations in the corpus
- An Introduction to 3D Bioprinting: Possibilities, Challenges and Future Aspects (2018, background): “Different types of tissues and organs have been produced using bioprinting, for instance; blood vessels [18], heart tissue [19], skin [20,21], liver tissue [5], neural tissue [22], cartilage [23] and bone [24].”
- Advances in engineering hydrogels (2017, background): “The emergence of microfluidic printheads capable of codelivering dual material types further enhanced the speed of multimaterial printing via improved switching between the different materials (118, 119, 130, 131).”