Phage selection restores antibiotic sensitivity in MDR Pseudomonas aeruginosa
By B. Chan, Mark J. Sistrom, J. Wertz, K. Kortright, D. Narayan, P. Turner. Scientific Reports, 2016
657 citations recorded by the source corpus.
Increasing prevalence and severity of multi-drug-resistant (MDR) bacterial infections has necessitated novel antibacterial strategies. Ideally, new approaches would target bacterial pathogens while exerting selection for reduced pathogenesis when these bacteria inevitably evolve resistance to therapeutic intervention. As an example of such a management strategy, we isolated a lytic bacteriophage, OMKO1, (family Myoviridae) of Pseudomonas aeruginosa that utilizes the outer membrane porin M (OprM) of the multidrug efflux systems MexAB and MexXY as a receptor-binding site. Results show that phage selection produces an evolutionary trade-off in MDR P. aeruginosa, whereby the evolution of bacterial resistance to phage attack changes the efflux pump mechanism, causing increased sensitivity to drugs from several antibiotic classes. Although modern phage therapy is still in its infancy, we conclude that phages, such as OMKO1, represent a new approach to phage therapy where bacteriophages exert selection for MDR bacteria to become increasingly sensitive to traditional antibiotics. This approach, using phages as targeted antibacterials, could extend the lifetime of our current antibiotics and potentially reduce the incidence of antibiotic resistant infections.
Subfields: Ecology, Microbiology, Molecular Biology
View Paper: https://www.semanticscholar.org/paper/ca470bfc12d3b22ae614c612ea94fd47d32076f8
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In Ecology
- Mansura S. Mulani
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- J. Barr
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Citations in the corpus
- Emerging Strategies to Combat ESKAPE Pathogens in the Era of Antimicrobial Resistance: A Review (2019, background): “Such an approach creates a win-win situation causing the killing of the bacteria either through phage or by antibiotic action (Chan et al., 2016).”
- Phage Therapy in the Postantibiotic Era (2019, background): “(140), demonstrated that applying the selective pressure of phage OMKO1 to a population of MDR P.”