Optimization of lignocellulolytic enzyme production by the white-rot fungus Trametes trogii in solid-state fermentation using response surface methodology
By L. Levin, C. Herrmann, V. L. Papinutti. 2008
202 citations recorded by the source corpus.
Abstract The white-rot basidiomicete Trametes trogii (MYA 28-11) is an outstanding producer of laccase. A Doehlert experimental design was applied to optimize its lignocellulolytic enzyme production in solid-state fermentation. The impact on enzyme production of three quantitative variables, namely pH, copper and nitrogen concentrations, was investigated by using a wood-based solid medium supplemented with malt extract. Optimization was aimed at simultaneously minimizing cellulase activity and maximizing ligninolytic enzyme production. Such conditions were: pH 4.5, peptone 12.5 g l −1 , and CuSO 4 11 mM. Highest activities achieved were: laccase 901 and Mn-peroxidase 20 U g −1 . There was not a direct correlation between the levels of enzymatic activities and the extent of losses of wood weight or components. The factorial approach also allowed us to quantify the enzyme activity in any part of the experimental domain, consequently, it was also possible to determine a culture medium to obtain crude extracellular extracts with high laccase (510 U g −1 ) and endoxylanase (780 U g −1 ) yields. These enzymes have gained renewed interest mainly due to their applications in paper industries for pulp treatment, improving the effectiveness of conventional bleaching. Considering the results obtained, T. trogii could be an attractive source of both enzymes.
Subfields: Biotechnology, Plant Science
View Paper: https://www.semanticscholar.org/paper/c991aad715042865de68a2b10923cd6896fa066a
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In Plant Science
Citations in the corpus
- Lignocellulose-degrading enzyme production by white-rot Basidiomycetes isolated from the forests of Georgia (2009, methodology): “In this respect, only few reports are concerned with the simultaneous production of hydrolytic and oxidative enzymes by white-rot fungi (Machuca and Ferraz 2001; Baldrian and Gabriel 2003; Nazareth and Sampy 2003; Sun et al. 2004; Kachlishvili et al. 2006; Elisashvili et al. 2006, 2008; Levin et al”
- Fungal Solid-State Fermentation and Various Methods of Enhancement in Cellulase Production (2014, unknown): “In general, an initial pH of approximately 5 is preferable for cellulase production by most of the WRF and BRF (Table 3) [9,50,55,58,62,67].”
- Advances in the Valorization of Lignocellulosic Materials by Biotechnology: An Overview (2013, background): “…et al. 2003; Couto and Sanromán 2005; Couto and Sanromán 2006; Eun et al. 2006; Papinutti and Forchiassin 2007; Papinutti and Lechner 2008; Levin et al. 2008; Oberoi et al. 2010; Stoilova et al. 2010; Asgher et al. 2011; Asgher and Iqbal 2011; Iqbal et al. 2011a; Yoon et al. 2012; Asgher…”