The long noncoding RNAs NEAT1 and MALAT1 bind active chromatin sites
By Jason A West, Christopher P. Davis, Hongjae Sunwoo, M. Simon, Ruslan I. Sadreyev, Peggy I. Wang, Michael Y. Tolstorukov, Robert E. Kingston. Molecules and Cells, 2014
667 citations recorded by the source corpus.
Summary Mechanistic roles for many lncRNAs are poorly understood in part because their direct interactions with genomic loci and proteins are difficult to assess. Using a method to purify endogenous RNAs and their associated factors, we mapped the genomic binding sites for two highly expressed human lncRNAs, NEAT1 and MALAT1. We show that NEAT1 and MALAT1 localize to hundreds of genomic sites in human cells, primarily over active genes. NEAT1 and MALAT1 exhibit colocalization to many of these loci, but display distinct gene body binding patterns at these sites, suggesting independent but complementary functions for these RNAs. We also identified numerous proteins enriched by both lncRNAs, supporting complementary binding and function, in addition to unique associated proteins. Transcriptional inhibition or stimulation alters localization of NEAT1 on active chromatin sites, implying that underlying DNA sequence does not target NEAT1 to chromatin and that localization responds to cues involved in the transcription process.
Subfields: Cancer Research, Endocrinology, Molecular Biology
View Paper: https://www.semanticscholar.org/paper/c6de9ca5bf4efffad254f93903b1d15081adb78c
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Citations in the corpus
- Systematic discovery of Xist RNA binding proteins (2015, methodology): “We also overlapped the set of proteins retrieved by Xist with those retrieved by two other abundant nuclear lncRNAs NEAT1 and MALAT1, and found limited overlap (14 out of 81 shared by all three, Figure S2C) (West et al., 2014).”
- Linking Long Noncoding RNA Localization and Function. (2016, methodology): “There are approximately 5–20 paraspeckles in each nucleus of most mammalian cells and NEAT1 is required for the integrity of paraspeckles [30–33].”
- Landscape of somatic mutations in 560 breast cancer whole genome sequences (2016, neutral): “Second, the role of driver substitutions and indels in non-coding regions of the genome20,21.”