Optimization of hydrolytic and oxidative enzyme methods for ecosystem studies

By D. German, M. Weintraub, A. S. Grandy, C. Lauber, Zachary L. Rinkes, S. Allison. 2011

1,205 citations recorded by the source corpus.

View Paper: https://www.semanticscholar.org/paper/c6cd7f742d3c12946f43aa3ef9c473dc68e92ae5

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In Pollution

In Soil Science

Citations in the corpus

Spatio-temporal patterns of enzyme activities after manure application reflect mechanisms of niche differentiation between plants and microorganisms (2017, background): “b-glucosidase is responsible for catalyzing the hydrolysis of terminal 1,4-linked bD-glucose residues from b-D-glucosides (German et al., 2011) and is”
Constraints to native plant species establishment in coastal dune communities invaded by Carpobrotus edulis : Implications for restoration (2013, methodology): “As recommended by German et al. (2011), we ran the enzyme assays at the environmental pH.”
Nitrogen inputs accelerate phosphorus cycling rates across a wide variety of terrestrial ecosystems. (2012, neutral): “One concern is that phosphatase is generally measured in the laboratory at standardized pH; however, optimal pH varies between enzymes and soils (Turner, 2010; German et al., 2011).”
Stability and dynamics of enzyme activity patterns in the rice rhizosphere: Effects of plant growth and temperature (2017, background): “, chitinase), which accomplishes the decomposition of chitin to a low molecular weight chitooligomer (German et al., 2011; Hoang et al., 2016a), at two temperatures (18 and 25 C) after 14 and 30 days.”
Functional guild classification predicts the enzymatic role of fungi in litter and soil biogeochemistry (2015, methodology): “Leucine amino peptidase (LAP) has semi-broad catalytic capabilities, catalyzing the hydrolysis of various amino acid residues (including leucine) as well as amino acid amides and methyl esters (German et al., 2011).”
Multiple phosphorus acquisition strategies adopted by fine roots in low-fertility soils in Central Amazonia (2019, methodology): “Root phosphatase activity Potential root acid phosphomonoesterase activity (APase) was measured for roots from 0 to 10 cm soil depth (n = 32) using a fluorimetric microplate assay adapted following protocols by German et al. (2011) and Turner and Romero (2010).”
Extracellular enzymes in terrestrial, freshwater, and marine environments: perspectives on system variability and common research needs (2013, background): “potential enzyme activities (Hoppe 1983; Somville and Billen 1983; German et al. 2011). These substrate 14 Biogeochemistry (2014) 117:5–21”
Using microbial communities and extracellular enzymes to link soil organic matter characteristics to greenhouse gas production in a tidal freshwater wetland (2014, neutral): “(2006) and German et al. (2011) with reagents”
Life in Dry Soils: Effects of Drought on Soil Microbial Communities and Processes (2018, background): “…measure potential enzyme activity using artificial substrates under optimal conditions (including adequate moisture) rather than field conditions (German et al. 2011); therefore, we have good reason to suspect that enzyme potential measurements may not reliably reflect in situ activity (Schimel…”
Active microorganisms in soil: Critical review of estimation criteria and approaches (2013, background): “Under substrate limitation common for soils, the apparent Km (as a result of simultaneous activity of multiple enzymes from both active and “stabilized” pools) is a preferable indicator of enzymes properties (German et al., 2011).”
The Michaelis–Menten kinetics of soil extracellular enzymes in response to temperature: a cross‐latitudinal study (2012, methodology): “Soil dry mass was determined following German et al. (2011a).”