Non-fermentative pathways for synthesis of branched-chain higher alcohols as biofuels

By S. Atsumi, T. Hanai, James C. Liao. Nature, 2008

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Industry 5.0—The Relevance and Implications of Bionics and Synthetic Biology (2016, background): “With the help of phototrophic or future heterotrophic organisms—so-called bioreactors—second-generation biofuels will use the whole plant and not just 4% of it as is used today [9,10].”
Multigene Editing in the Escherichia coli Genome via the CRISPR-Cas9 System (2015, background): “Metabolic engineering is widely applied to modify Escherichia coli to produce industrially relevant biofuels or biochemicals, including ethanol (1), higher alcohols (2), fatty acids (3), amino acids (4), shikimate precursors (5), terpenoids (6), polyketides (7), and polymeric precursors of 1,4-butan”
Synthetic biology: applications come of age (2010, background): “In an altogether different approach, Liao and colleague”
The economics of current and future biofuels (2009, background): “Newly engineered Escherichia coli strains as the microbial cultures have been developed to produce isobutanol (Atsumi et al. 2007, 2008; Hanai et al. 2007), in addition to n-butanol production.”
Direct photosynthetic recycling of carbon dioxide to isobutyraldehyde (2009, background): “To increase the flux to the keto acid precursor, 2-ketoisovalerate (KIV”