Estimating prokaryotic diversity: When are 16S rDNA libraries large enough?

By P. Kemp, J. Aller. 2004

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Effects of salinity and nutrients on microbial assemblages in Louisiana wetland sediments (2009, methodology): “This demonstrates that bacterial diversity estimates are not necessarily accurate; a result of limited sampling of dominant bacterial populations, and true bacterial diversity in most aquatic environments is much higher (Kemp and Aller 2004b).”
The Road to Metagenomics: From Microbiology to DNA Sequencing Technologies and Bioinformatics (2015, background): “However, in nature, the microbial communities have a large number of rare species that can be detected only if an exhaustive sampling is performed (Colwell and Coddington, 1994; Kemp and Aller, 2004; Bonilla-Rosso et al., 2012).”