Double-stranded RNA interferes with gene function at the single-cell level in cereals
By P. Schweizer, J. Pokorny, Paul Schulze-Lefert, R. Dudler. The Plant Journal, 2000
186 citations recorded by the source corpus.
Double-stranded RNA (dsRNA) has been shown to specifically interfere with gene function in several organisms including tobacco and the model plant Arabidopsis. Here, we report on rapid and sequence-specific interference of dsRNA with gene function in cereals. Delivery of cognate dsRNA into single epidermal cells of maize, barley or wheat by particle bombardment interfered with the function of co-bombarded UidA (GUS) and TaGLP2a:GFP reporter genes. Cognate dsRNA was also found to specifically interfere with the function of the endogenous genes A1 and Ant18 encoding dihydroflavonol-4-reductase in maize and barley, respectively. Dihydroflavonol-4-reductase is an essential enzyme of the anthocyanin biosynthetic pathway in maize and barley. This pathway can be induced by transient expression of the C1- and b-Peru genes that encode transcription factors. In the presence of dsRNA corresponding to the dihydroflavonol-4-reductase gene, C1- and b-Peru-dependent, cell-autonomous accumulation of red anthocyanin pigments in bombarded cells of maize and barley was reduced. dsRNA was also demonstrated to negatively interfere with Mlo, which encodes a negative regulator of race non-specific resistance to the powdery mildew fungus in barley. In the presence of Mlo dsRNA, transformed cells became more resistant, thereby phenocopying plants that carry a heritable loss-of function mlo resistance allele. The results suggest that direct delivery of dsRNA to cereals leads to a rapid and sequence-specific interference with gene function at the single-cell level.
Subfields: Endocrinology, Plant Science
View Paper: https://www.semanticscholar.org/paper/c420479d1b8f7dbace0badf5bc5c0eb29061ef60
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Citations in the corpus
- HIGS: Host-Induced Gene Silencing in the Obligate Biotrophic Fungal Pathogen Blumeria graminis[W][OA] (2010, background): “…transient overexpression of a synthetic Avra10 gene induced cell death in the presence ofMla10 (Pallas near-isogenic line P09), which was reflected by a reduced number of epidermal cells accumulating anthocyanin induced by the cobombarded reporter plasmid pBC17 (Schweizer et al., 2000) (Figure 5B).”
- A practical vector for efficient knockdown of gene expression in rice (Oryza sativa L.) (2004, unknown): “However, the tedious procedure of the special construction to ensure the formation of a hairpin structure significantly limits the application of the technology in the plant kingdom (Chuang and Meyerowitz, 2000; Schweizer et al., 2000).”