Vouchering DNA-barcoded specimens: test of a nondestructive extraction protocol for terrestrial arthropods

By D. Rowley, Jonathan Coddington, M. Gates, A. Norrbom, R. Ochoa, N. Vandenberg, M. Greenstone. 2007

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Current progress in DNA barcoding and future implications for entomology (2011, background): “…have been proposed (Johnson & Clayton 2003: lice; Favret 2005: aphids; Pons 2006; Gilbert et al. 2007: Coleoptera; Petersen et al. 2007: tarantulas; Rowley et al. 2007: Acarina, Araneae, Coleoptera, Diptera and Hymenoptera; Badek et al. 2008: analgoid mites; Hunter et al. 2008: Diptera; Katoh et…”
A hands-on overview of tissue preservation methods for molecular genetic analyses (2010, methodology): “Special attention is required for type specimens, where the top priority is to minimize damage to the specimen, and therefore less invasive methods of tissue sampling should be favoured and applied: biopsy-like methods, removal of parts not used for taxonomic diagnosis, or minimally invasive samplin”
A rapid non-destructive DNA extraction method for insects and other arthropods (2010, methodology): “In contrast, the previously described non-destructive method to extract DNA from terrestrial arthropods (Rowley et al., 2007) required more than 4 h.”
Applying molecular-based approaches to classical biological control of weeds (2011, background): “Development of noninvasive DNA extraction methods for preserved arthropods has recently made considerable progress (e.g. Rowley et al., 2007; Hunter et al., 2008) because DNA from specimens can be induced to leak into extraction buffers (Gilbert et al., 2007).”