ORS1, an H2O2-Responsive NAC Transcription Factor, Controls Senescence in Arabidopsis thaliana
By S. Balazadeh, Miroslaw Kwasniewski, Camila Caldana, M. Mehrnia, M. Zanor, G. Xue, B. Mueller‐Roeber. Molecular Plant, 2011
303 citations recorded by the source corpus.
We report here that ORS1, a previously uncharacterized member of the NAC transcription factor family, controls leaf senescence in Arabidopsis thaliana. Overexpression of ORS1 accelerates senescence in transgenic plants, whereas its inhibition delays it. Genes acting downstream of ORS1 were identified by global expression analysis using transgenic plants producing dexamethasone-inducible ORS1–GR fusion protein. Of the 42 up-regulated genes, 30 (∼70%) were previously shown to be up-regulated during age-dependent senescence. We also observed that 32 (∼76%) of the ORS1-dependent genes were induced by long-term (4 d), but not short-term (6 h) salinity stress (150 mM NaCl). Furthermore, expression of 16 and 24 genes, respectively, was induced after 1 and 5 h of treatment with hydrogen peroxide (H2O2), a reactive oxygen species known to accumulate during salinity stress. ORS1 itself was found to be rapidly and strongly induced by H2O2 treatment in both leaves and roots. Using in vitro binding site selection, we determined the preferred binding motif of ORS1 and found it to be present in half of the ORS1-dependent genes. ORS1 is a paralog of ORE1/ANAC092/AtNAC2, a previously reported regulator of leaf senescence. Phylogenetic footprinting revealed evolutionary conservation of the ORS1 and ORE1 promoter sequences in different Brassicaceae species, indicating strong positive selection acting on both genes. We conclude that ORS1, similarly to ORE1, triggers expression of senescence-associated genes through a regulatory network that may involve cross-talk with salt- and H2O2-dependent signaling pathways.
Subfields: Molecular Biology, Plant Science
View Paper: https://www.semanticscholar.org/paper/c2eb15c9d4302b777bdadbd12489eb58ccf6f6fa
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Citations in the corpus
- The Complex Regulation of Senescence (2012, background): “A paralogous gene (termed ORESARA1 SISTER1 or ORS1) with important functions in Arabidopsis senescence regulation has recently been characterized by Balazadeh et al. (2011).”
- Comprehending crystalline β-carotene accumulation by comparing engineered cell models and the natural carotenoid-rich system of citrus (2011, unknown): “The transcript abundance was normalized against peanut Ubiquitin (Luo et al. , 2005) transcript levels.”