Development, characterization and utilization of microsatellite markers in pigeonpea
By D. Odeny, B. Jayashree, M. Ferguson, David A. Hoisington, J. Crouch, C. Gebhardt. Plant Breeding, 2007
114 citations recorded by the source corpus.
Pigeonpea is a major legume of the semi-arid tropics that has been neglected in terms of molecular breeding. The objectives of this study were to develop microsatellite markers and evaluate their potential for use in pigeonpea genetics and breeding. Two hundred and eight microsatellite loci were isolated by screening a non-enriched partial genomic library. Primers were designed for 39 microsatellite loci, 20 of which amplified polymerase chain reaction products of the expected size. Nineteen of the primer pairs were polymorphic amongst 15 cultivated and nine wild pigeonpea accessions providing evidence for cross-species transferability within the genus Cajanus. A total of 98 alleles were detected at the 19 polymorphic loci with an average of 4.9 alleles per locus. The observed heterozygosity ranged from 0.17 to 0.80 with a mean of 0.42 per locus. Less allelic variation (31 alleles) was observed within the cultivated species than across the wild species (92 alleles). The diversity analysis readily distinguished all wild relatives from each other and from the cultivated germplasm. Development of more microsatellites is recommended for future genomic studies in pigeonpea.
Subfields: Plant Science
View Paper: https://www.semanticscholar.org/paper/bed707efbacf76d36a2035e09527dd89e136056d
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In Plant Science
Citations in the corpus
- Genetic Improvement of Pigeon Pea — A Review (2008, background): “These markers (19 polymorphic loci) yielded an average of 4.9 alleles per locus while the observed heterozygosity ranged from 0.17–0.80 with a mean of 0.42 per locus [ 66 ].”
- Application of SSR markers for molecular characterization of hybrid parents and purity assessment of ICPH 2438 hybrid of pigeonpea [Cajanus cajan (L.) Millspaugh] (2010, background): “…as mentioned above, in the recent past some SSR-marker-based genetic diversity studies have been undertaken in pigeonpea (Burns et al. 2001; Odeny et al. 2007; Saxena et al. 2010a,b), SSR markers have not yet been exploited to assess diversity within and among A, B, and R lines of A4 (C.…”
- A preliminary genetic analysis of fibre traits and the use of new genomic SSRs for genetic diversity in jute (2008, background): “Therefore, in recent years, considerable emphasis has been placed on the development and use of molecular markers in all major crops (Liu et al. 1996; Röder et al. 1998; McCouch et al. 1997; Sharopova et al. 2002; Hirata et al. 2006; Odeny et al. 2007).”
- Novel SSR Markers for Polymorphism Detection in Pigeonpea (Cajanus spp.) (2010, background): “Indeed, similar kind of reports on observations of less genetic variation within cultivated gene pool as compared with inter-specific gene pool have been reported in past using SSR (Odeny et al. 2007) and diversity array technology (DArT) markers (Yang et al. 2006).”