Identification of Meloidogyne incognita, M. javanica and M. arenaria using sequence characterised amplified region (SCAR) based PCR assays

By C. Zijlstra, Dorine Donkers-venne, M. Fargette. 2000

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The reproductive potential of the root-knot nematode Meloidogyne incognita is affected by selection for virulence against major resistance genes from tomato and pepper (2011, methodology): “Prior to multiplication, each isolate was specifically identified according to its isoesterase electrophoretic pattern (Dalmasso and Bergé 1978) and/or by SCARPCR (Zijlstra et al. 2000).”
Molecular diagnostic key for identification of single juveniles of seven common and economically important species of root‐knot nematode (Meloidogyne spp.) (2007, result): “arenaria) (Zijlstra et al., 2000) gave very consistent results and the products were readily amplified from single individual juvenile nematodes (Fig.”
Pathogenicity of the root‐knot nematode Meloidogyne javanica on potato (2005, methodology): “Similarly, PCR assays (with primer set Fj /Rj) using DNA extracted from potato nematodes in Malta and from the M. javanica reference isolate from peach gave rise to the M. javanica marker amplicon of 670 bp (Zijlstra et al., 2000) (Fig.”
Effects on plant growth and root-knot nematode infection of an endophytic GFP transformant of the nematophagous fungus Pochonia chlamydosporia (2012, neutral): “Root-knot nematodes (RKN) M. javanica were from a field population (Almeria, SE Spain) PCR identified as in Zijlstra et al. (2000).”