PCR-based sex determination in Odontocete cetaceans

By P. Rosel. Conservation Genetics, 2003

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Mitochondrial and nuclear DNA analyses reveal fine scale geographic structure in bottlenose dolphins (Tursiops truncatus) in the Gulf of Mexico (2005, methodology): “Samples collected via biopsy darting were sexed using ZFX and SRY specific primers as described in Rosel (2003) .”
Songs of male humpback whales, Megaptera novaeangliae, are involved in intersexual interactions (2008, unknown): “We extracted the DNA using an ammonium acetate method (Nicholls et al. 2000) and amplified it using a multiplex PCR method similar to that described in Rosel (2003), using primers P15EZ, P23EZ for the Zfx/Zfy gene (Aasen & Medrano 1990) and Y53-3c, Y53-3d for the SRY gene (Gilson et al. 1998).”
Mitochondrial Control Region and microsatellite analyses on harbour porpoise (Phocoena phocoena) unravel population differentiation in the Baltic Sea and adjacent waters (2010, methodology): “Samples of unknown gender were Polymerase Chain Reaction (PCR) sexed using ZFX and SRY specific primers; PCR conditions were as in Rosel (2003). Conserv Genet (2010) 11:195–211 197”