Multiplexed coded illumination for Fourier Ptychography with an LED array microscope.

By Lei Tian, Xiao Li, K. Ramchandran, Laura Waller. Biomedical Optics Express, 2014

553 citations recorded by the source corpus.

Fourier Ptychography is a new computational microscopy technique that achieves gigapixel images with both wide field of view and high resolution in both phase and amplitude. The hardware setup involves a simple replacement of the microscope's illumination unit with a programmable LED array, allowing one to flexibly pattern illumination angles without any moving parts. In previous work, a series of low-resolution images was taken by sequentially turning on each single LED in the array, and the data were then combined to recover a bandwidth much higher than the one allowed by the original imaging system. Here, we demonstrate a multiplexed illumination strategy in which multiple randomly selected LEDs are turned on for each image. Since each LED corresponds to a different area of Fourier space, the total number of images can be significantly reduced, without sacrificing image quality. We demonstrate this method experimentally in a modified commercial microscope. Compared to sequential scanning, our multiplexed strategy achieves similar results with approximately an order of magnitude reduction in both acquisition time and data capture requirements.

Subfields: Astronomy and Astrophysics, Atomic and Molecular Physics, and Optics, Radiation

View Paper: https://www.semanticscholar.org/paper/bd5853e441e81a07d22dbc3d22b06622a08bcf36

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Citations in the corpus

3D intensity and phase imaging from light field measurements in an LED array microscope (2015, methodology): “This simple, inexpensive hardware modification enables not only 4D light field capture [35,38], but also dark field [38,39], phase contrast [35,39], Fourier ptychography [23,24], and digital aberration removal [40].”
Computational illumination for high-speed in vitro Fourier ptychographic microscopy (2015, methodology): “Previous work in FPM uses a low-resolution intensity image as the initial guess [7,15,16,31].”
Experimental robustness of Fourier Ptychography phase retrieval algorithms (2015, methodology): “The original FPM algorithm used a Gerchberg-Saxton approach [9], which is a type of alternating projections [10–13], first developed for traditional ptychography [2, 3, 14–17] and later for FPM [1,18,19].”
Phase Retrieval via Wirtinger Flow: Theory and Algorithms (2014, methodology): “Because of the practical significance of the phase retrieval problem in imaging science, the community has developed methods for recovering a signal x ∈ Cn from data of the form yr = ∣⟨ar,x⟩∣2 in the special case where one samples the (square) modulus of its Fourier transform.”