The Protocol for SNAC-tag Chemical Protein Cleavage

By Bobo Dang, William F. DeGrado. 2019

This protocol describes a method to site-specifically cleave proteins using chemicals upon insertion of the appropriate tag into the cleaving proteins. It can be applied to water soluble as well as membrane proteins with efficiency comparable to enzymes. The fact that chemicals can be used to replace enzymes in this method highlights the unique aspects of this technology. The cleavage can be achieved within a day upon obtaining target proteins. Detailed technical aspects of this method will be discussed in this protocol.