Rapid, transient expression of fluorescent fusion proteins in tobacco plants and generation of stably transformed plants

By I. Sparkes, J. Runions, A. Kearns, C. Hawes. Nature Protocols, 2006

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Transient Expression of (2011, background): “Sparkes et al (2006) showed that transient transformation of tobacco leaf epidermal cells is a relatively fast technique to assess the expression of genes of interest.”