Interfacial adsorption of lipases on very hydrophobic support (octadecyl-Sepabeads): Immobilization, hyperactivation and stabilization of the open form of lipases

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Glyoxyl agarose: A fully inert and hydrophilic support for immobilization and high stabilization of proteins (2006, background): “Thus, many enzymes have been highly stabilized by immobilization on this support (Table 1): [17–43] activity recoveries ranged from 60 to 100%, while stabilizations (usually increments in the half-lives) in most cases were higher than 103 and in certain cases reached values of 105.”
Fungal lipases : a review (2017, background): “It is also easier to control reaction parameters like flow rate and accessibility of substrates when the enzyme is immobilized [126, 127].”
Strategies for the one-step immobilization-purification of enzymes as industrial biocatalysts. (2015, methodology): “A special casewill be the immobilization of lipases on hydrophobic supports via interfacial activation, which produces some stabilization (Palomo et al., 2002).”
Immobilization of Enzymes and Cells (2006, positive): “Usually methyltrimethoxysilane CH3Si(OCH3)3 (MTMS) was used as the precursor and polyvinyl alcohol (PVA) as an additive, the latter possibly acting as a stabilizer of the lipase (17,18).”