In vitro induction of tetraploid ginger (Zingiber officinale Roscoe) and its pollen fertility and germinability

By S. Adaniya, Daisuke Shirai. 2001

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Mitotic chromosome doubling of plant tissues in vitro (2010, background): “…Colchicine 5 mM, 12 h Gao et al. (2002) Zingiber officinale Shoot tips, solid or liquid medium with colchicine Colchicine 5 mM, 8 d (solid medium) Adaniya and Shirai (2001) a d days, w weeks Besides the treatment of buds or shoots with mitotic inhibitors in the tissue culture medium (liquid or…”
In vitro induction of tetraploids in Phlox subulata L. (2007, background): “…one of prevalent methods of polyploidy induction, which was successfully applied to a number of plant species, including Pyrus pyrifolia (Kadota and Niimi 2002), Zingiber officinale (Adaniya and Shirai 2001), Punica granatum (Shao et al. 2003), Alocasia (Thao et al. 2003), and Zizyphus jujuba Mill.”
Morphological, physiological, cytological and phytochemical studies in diploid and colchicine-induced tetraploid plants of Echinacea purpurea (L.) (2013, background): “Increasing the levels of secondary metabolites as a result of polyploidy induction has been reported for some medicinal plants (Adaniya and Shira 2001; Berkov and Philipov 2002; Gonzalez and Weathers 2003; Majdi et al. 2010).”