Flow Cytometry Analysis of Marine Picoplankton

By Dominique Marie, N. Simon, Laure Guillou, Frédéric Partensky, Daniel Vaulot. 2000

145 citations recorded by the source corpus.

Subfields: Ecology, Molecular Biology, Oceanography

View Paper: https://www.semanticscholar.org/paper/b8baa1b4d8058815768a8faa20cead39a19420cd

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Citations in the corpus

Nutrient limitation of primary productivity in the Southeast Pacific (BIOSOPE cruise) (2007, background): “Populations were differentiated based on their scattering and fluorescence signals (Marie et al., 2000).”
Lack-of P-limitation of phytoplankton and heterotrophic prokaryotes in surface waters of three anticyclonic eddies in the stratified Mediterranean Sea (2011, background): “5% for the former and 1% for the latter), frozen in liquid nitrogen, and stored at −80C until analysis (e.g., Troussellier et al., 1995; Marie et al., 2000; Lebaron et al., 2001; Brussaard, 2004).”
Culturing the marine cyanobacterium Prochlorococcus (2007, methodology): “Comparison of final cell yield was quantified flow cytometrically with a FACS-Scan or FACS-Calibur (BD Biosciences) following standard methods (Marie et al. 2000).”
Microbial community structure in the sea surface microlayer at two contrasting coastal sites in the northwestern Mediterranean Sea (2006, background): “Stained bacteria were counted according to their right angle light scatter and green fluorescence measured at 515 to 545 nm (Marie et al. 1997, 2000).”
Picophytoplankton cell death induced by UV radiation: Evidence for oceanic Atlantic communities (2006, methodology): “…of Prochlorococcus and Synechococcus cells during the experiments was determined on-board by flow cytometric analysis of duplicated fresh samples with a FACSCALIBUR flow cytometer (Becton Dickinson) according to the characteristic features of fluorescence for each population (Marie et al. 1999).”
Contribution of picoplankton to the total particulate organic carbon concentration in the eastern South Pacific (2007, methodology): “The data were then analysed with the Cytowin software (Vaulot, 1989) to separate the picoplanktonic populations based on their scattering and fluorescence signals, according to Marie et al. (2000) (see Supp.”
Routine quantification of phytoplankton groups— microscopy or pigment analyses? (2004, methodology): “…(0.05 and 1% final concentration, respectively, see Jacquet et al. 1998a) for 15 min in dim light conditions, frozen in liquid nitrogen and kept at –80°C until flow-cytometry analysis was performed with a FACSort instrument (Becton Dickinson) as described in Marie et al. (1999, 2000).”
Alkaline phosphatase activity and regulation in the North Pacific Subtropical Gyre (2010, unknown): “Samples were processed according to Marie et al. (2000).”