Diversity and phylogenetic profiling of niche-specific Bacilli from extreme environments of India
By Ajar Nath Yadav, P. Verma, Murugan Kumar, K. K. Pal, R. Dey, Alka Gupta, J. Padaria, G. Gujar, Sudheer Kumar, A. Suman and 2 more. Annals of Microbiology, 2014
149 citations recorded by the source corpus.
The diversity of culturable, aerobic and heterotrophic Bacillus and Bacillus-derived genera (BBDG) was investigated in various extreme environments (including thermal springs, cold deserts, mangroves, salt lakes, arid regions, salt pans and acidic soils) of India. Heat treatment followed by enrichment in different media led to a total of 893 bacterial isolates. Amplified ribosomal DNA restriction analysis (ARDRA) using three restriction enzymes AluI, MspI and HaeIII led to the clustering of these isolates into 12–74 groups for the different sites at 75 % similarity index, adding up to 559 groups. Phylogenetic analysis based on 16S rRNA gene sequencing led to the identification of 392 bacilli, grouped in two families, Bacillaceae (89.03 %) and Paenibacillaceae (10.97 %), and included 13 different genera with 75 distinct species. It was found that among the thirteen genera, nine (Bacillus, Halobacillus, Lysinibacillus, Oceanobacillus, Pontibacillus, Salinibacillus, Sediminibacillus, Thalassobacillus and Virgibacillus) belonged to Bacillaceae and four (Ammoniphilus, Aneurinibacillus, Brevibacillus and Paenibacillus) belonged to Paenibacillaceae. Novel isolates tolerant to low and high pH and temperature, salt and low moisture were identified. The major outcome of the present investigation was the identification of niche-specific species and also the ubiquitous presence of selected species of BBDG, which illustrate the diversity and pervasive nature of BBDG in extreme environments.
Subfields: Ecology, Food Science, Molecular Biology
View Paper: https://www.semanticscholar.org/paper/b86ec6f93e4c33e57ecdc0ce95a4df9b0072ed37
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Citations in the corpus
- Plant Microbiomes and Its Beneficial Multifunctional Plant Growth Promoting Attributes (2017, background): “The study on microbial biodiversity of plant associated microbes revealed representative microbes from archaea (Euryarchaeota); bacteria (Acidobacteria, Actinobacteria, Bacteroidetes, Deinococcus-Thermus, Firmicutes and Proteobacteria) and fungi (Ascomycota and Basidiomycota).”
- Plant Growth Promoting Bacteria: Biodiversity and Multifunctional Attributes for Sustainable Agriculture (2017, methodology): “The microbes could be isolated using enrichment and serial dilution methods followed by spread or pour plates technique [18].”
- Evaluating the diversity and phylogeny of plant growth promoting bacteria associated with wheat (Triticum aestivum) growing in central zone of India (2014, methodology): “All the isolates were screened in triplicates for tolerance to pH and drought [Low water potential on polyethylene glycol (PEG8000) - infused plates] as described earlier Yadav et al. (2014).”
- Biodiversity and biotechnological applications of halophilic microbes for sustainable agriculture (2018, background): “The salt-loving microbes can be grouped as true halophiles (15-30% NaCl), moderate halophiles (3-15% M NaCl), and slight halophiles (1-2% NaCl) [1].”