Highly inclined thin illumination enables clear single-molecule imaging in cells

By M. Tokunaga, N. Imamoto, K. Sakata-Sogawa. Nature Methods, 2008

1,260 citations recorded by the source corpus.

Subfields: Biophysics, Structural Biology

View Paper: https://www.semanticscholar.org/paper/b84715c594136d4929697f6791070f3c776b0df8

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Citations in the corpus

Selective plane illumination microscopy techniques in developmental biology (2009, methodology): “…microscopy Single lens for oblique light-sheet illumination and detection Single lens Very narrow FOV Single cell microscopy close to the coverslip Tokunaga et al., 2008 Oblique plane microscopy (OPM) Like HILO, matching, tilted image plane Like HILO, extended FOV Extensive optics Like HILO…”
The light-sheet microscopy revolution (2018, background): “HILO or iSPIM Highly Inclined and laminated Optical sheet or inclined SPIM [105,106]”
Direct stochastic optical reconstruction microscopy with standard fluorescent probes (2011, background): “An interesting approach is the illumination by a highly inclined and thin beam that allows axial sectioning (highly inclined and laminated optical sheet, HILO”
Lattice Light Sheet Microscopy: Imaging Molecules to Embryos at High Spatiotemporal Resolution (2014, neutral): “Light-sheet microscopy has been applied to address this issue( 20 , 21 )butwithconventionalGaussianlight sheets that are, over cellular dimensions, at least ∼ twoto fivetimesthickerthan thedepthoffocus of a high-NA detection objective.”
Real-time imaging of DNA loop extrusion by condensin (2018, positive): “S12) and co-imaged them with SxO-labeled DNA in a Highly Inclined and Laminated Optical (HILO) sheet mode (26).”
Three-dimensional sub-100 nm super-resolution imaging of biological samples using a phase ramp in the objective pupil (2011, methodology): “Highly inclined plane illumination [ 13 ] was used with a 671 nm laser source, leading to a focal plane intensity of approximately 10 9 W/m 2 . The setup is described in more detail in [14].”
RNA controls PolyQ protein phase transitions (2015, background): “TIRF was performed as previously described (Tokunaga et al., 2008).”
Rapid three-dimensional isotropic imaging of living cells using Bessel beam plane illumination (2011, neutral): “Highly inclined, objective-coupled sheet illuminatio”