Eleven Golden Rules of Quantitative RT-PCR

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Evaluation of coffee reference genes for relative expression studies by quantitative real-time RT-PCR (2009, background): “Normalization is necessary for the correction of technical variations such as differences in the quantity and quality of RNA, and in the efficiencies of reverse transcription and PCR (Udvardi et al. 2008).”
Validation of Candidate Reference Genes for the Accurate Normalization of Real-Time Quantitative RT-PCR Data in Rice During Seed Development (2010, background): “Using multiple stably-expressed reference genes is currently considered the gold standard for normalization (Gutierrez et al. 2008; Udvardi et al. 2008).”
Reference Gene Selection for Real-Time Quantitative Polymerase Chain Reaction of mRNA Transcript Levels in Chinese Cabbage (Brassica rapa L. ssp. pekinensis) (2010, background): “To overcome this limitation, researchers typically normalized the mRNA expression of their genes of interest to the expression of a constitutively expressed reference gene (Udvardi et al. 2008), and new reference genes are being considered, including synthetic templates and random oligoprimers (Phil”
Comprehending crystalline β-carotene accumulation by comparing engineered cell models and the natural carotenoid-rich system of citrus (2011, neutral): “In recent years, the plant scientific community has gradually recognized the need for robust validation (Gutierrez et al., 2008a, b; Udvardi et al., 2008; Guenin et al., 2009).”