Extraction of high molecular weight DNA from molluscs.

By B. Winnepenninckx, Thierry Backeljau, R. D. Wachter. 2002

936 citations recorded by the source corpus.

Subfields: Ecology, Global and Planetary Change

View Paper: https://www.semanticscholar.org/paper/b67d2d5fa0d6d523fbd04100fc3ad2d285c19e37

How researchers see this paper

The researchers whose trusted networks rank it highest, strongest advocate first. Every researcher gets a different order.

In Ecology

1. C. Albrecht
2. Peer Bork
3. B. Streit
4. S. Trajanovski
5. K. Kuhn
6. Jeroen Raes
7. J. Ducroz
8. Gipsi Lima-Mendez

In Global and Planetary Change

1. J. Ducroz
2. J. Grahame
3. Z. Nagy
4. C. Wilding
5. L. Granjon
6. R. Butlin
7. V. Volobouev

Citations in the corpus

• Parasite-responsive IgSF members in the snail Biomphalaria glabrata: characterization of novel genes with tandemly arranged IgSF domains and a fibrinogen domain (2001, methodology): “Snail genomic DNA extraction was carried out according to a CTAB protocol ( Winnepenninckx et al. 1993 ).”
• Timing of the population dynamics of bullhead Cottus gobio (Teleostei: Cottidae) during the Pleistocene (2002, methodology): “The tissue was incubated overnight in cetyltrimethylammonium bromide (CTAB)-buffer [2% CTAB, 1.4 M NaCl, 0.2% b-mercaptoethanol, 20 mM ethylenediaminetetraacetic acid (EDTA) and 100 mM Tris pH 8.0] at 55 C (Winnepenninckx et al., 1993).”
• The roles of rivers and Pleistocene refugia in shaping genetic diversity in Praomys misonnei in tropical Africa (2011, methodology): “DNA was extracted from ethanol-preserved tissues by the cetyl trimethyl ammonium bromide (CTAB) method (Winnepenninckx et al., 1993).”
• Evolutionary history of the Paradoxurus palm civets – a new model for Asian biogeography (2010, methodology): “Total genomic DNA was isolated using a cetyl trimethyl ammonium bromide (CTAB)-based protocol (Winnepenninckx et al., 1993) and a QIAamp DNA microkit (Qiagen, Illkirch, France) from fresh and museum samples, respectively.”
• An Assessment of the Systematics of Arvicanthine Rodents Using Mitochondrial DNA Sequences: Evolutionary and Biogeographical Implications (2001, methodology): “The DNA extraction was performed following the CTAB method as described in Winnepenninckx et al. (1993).”
• Rapid evolution of an ancient lake species flock: Freshwater limpets (Gastropoda: Ancylidae) in the Balkan Lake Ohrid (2006, methodology): “DNA from ethanol-preserved snails was extracted utilizing the protocol of Winnepenninckx et al. (1993).”
• Exploring nucleo-cytoplasmic large DNA viruses in Tara Oceans microbial metagenomes (2013, methodology): “DNA was extracted using a modified CTAB (hexadecyltrimethylammonium bromide) protocol (Winnepenninckx et al., 1993): (i) the filters were incubated at 60 1C for 1 h in a CTAB buffer (2% CTAB; 100 mM TrisHCl (pH¼ 8); 20 mM EDTA; 1.4 M NaCl; 0.2% b-mercaptoethanol; 0.1 mg ml 1 proteinase K; 10 mM DTT…”
• A hands-on overview of tissue preservation methods for molecular genetic analyses (2010, background): “It is mainly used for plant samples, but also performs well on animal tissues or protozoan cells (e.g. Shahjahan et al. 1995; Winnepenninckx et al. 1993).”