The Landscape of Circular RNA in Cancer

By Josh N. Vo, M. Cieslik, Yajia Zhang, S. Shukla, Lanbo Xiao, Yuping Zhang, Yi-Mi Wu, S. Dhanasekaran, Carl G. Engelke, Xuhong Cao and 3 more. Cell, 2019

1,367 citations recorded by the source corpus.

SUMMARY Circular RNAs (circRNAs) are an intriguing class of RNA due to their covalently closed structure, high stability, and implicated roles in gene regulation. Here, we used an exome capture RNA sequencing protocol to detect and characterize circRNAs across >2,000 cancer samples. When compared against Ribo-Zero and RNase R, capture sequencing significantly enhanced the enrichment of circRNAs and preserved accurate circular-to-linear ratios. Using capture sequencing, we built the most comprehensive catalogue of circRNA species to date: MiOncoCirc, the first database to be composed primarily of circRNAs directly detected in tumor tissues. Using MiOncoCirc, we identified candidate circRNAs to serve as biomarkers for prostate cancer and were able to detect circRNAs in urine. We further detected a novel class of circular transcripts, termed read-through circRNAs, that involved exons originating from different genes. MiOncoCirc will serve as a valuable resource for the development of circRNAs as diagnostic or therapeutic targets across cancer types.

Subfields: Cancer Research, Molecular Biology

View Paper: https://www.semanticscholar.org/paper/b67513a6e81f117c54be0f639e75adc7a2c9d870

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In Cancer Research

1. Wanying Wu
2. Peifeng Ji
3. Fangqing Zhao

In Molecular Biology

1. Wanying Wu
2. Peifeng Ji
3. Fangqing Zhao

Citations in the corpus

• CircAtlas: an integrated resource of one million highly accurate circular RNAs from 1070 vertebrate transcriptomes (2020, background): “…of large circRNA datasets and the imperative requirement of delineating their functional importance, various databases (e.g., circBase [17], CIRCpedia v2 [18], circRNADb [19], TSCD [20], CircRiC [21], and MiOncoCirc [7]) have been established to extend the catalog of the annotated circRNAs.”
• Circular RNA-protein interactions: functions, mechanisms, and identification (2020, background): “This stability is crucial and will likely prove an ideal property of circRNAs during their future development as biomarkers [10-13].”
• The bioinformatics toolbox for circRNA discovery and analysis (2020, background): “CSCD [120] and MiOncoCirc [17] are cancer-specific circRNA databases aiming to facilitate the functional study of cancer-specific circRNAs, while HDncRNA [121] and LncRNADisease [122] store disease-associated ncRNAs including circRNAs.”