Direct ubiquitination of pattern recognition receptor FLS2 attenuates plant innate immunity

By Dongping Lu, Wenwei Lin, Xiquan Gao, Shujing Wu, Cheng Cheng, Julian Avila, Antje Heese, T. Devarenne, Ping He, Libo Shan. Nature reviews. Immunology, 2011

612 citations recorded by the source corpus.

Targeted degradation of bacterial sensing proteins keeps plant defenses from running amok. Innate immune responses are triggered by the activation of pattern-recognition receptors (PRRs). The Arabidopsis PRR FLAGELLIN-SENSING 2 (FLS2) senses bacterial flagellin and initiates immune signaling through association with BAK1. The molecular mechanisms underlying the attenuation of FLS2 activation are largely unknown. We report that flagellin induces recruitment of two closely related U-box E3 ubiquitin ligases, PUB12 and PUB13, to FLS2 receptor complex in Arabidopsis. BAK1 phosphorylates PUB12 and PUB13 and is required for FLS2-PUB12/13 association. PUB12 and PUB13 polyubiquitinate FLS2 and promote flagellin-induced FLS2 degradation, and the pub12 and pub13 mutants displayed elevated immune responses to flagellin treatment. Our study has revealed a unique regulatory circuit of direct ubiquitination and turnover of FLS2 by BAK1-mediated phosphorylation and recruitment of specific E3 ligases for attenuation of immune signaling.

Subfields: Plant Science

View Paper: https://www.semanticscholar.org/paper/b50b50e1874dfbc3768d52ffe8085b0e496c5819

How researchers see this paper

The researchers whose trusted networks rank it highest, strongest advocate first. Every researcher gets a different order.

In Plant Science

1. Cyril Zipfel
2. Alok Kumar Pandey
3. Subhadeep Chatterjee
4. Ping He
5. Libo Shan
6. Jacqueline Monaghan

Citations in the corpus

• Plant Pattern Recognition Receptors (2017, methodology): “Using in vitro ubiquitination assays, the purified cytosolic domain of FLS2 was shown to be ubiquitinated by both the Pseudomonas syringae effector AvrPtoB [9] and the plant E3 ligases PUB12 and PUB13 [10], which results in its degradation in vivo.”
• Signaling in Plant-Microbe Interactions (2013, background): “It also indicates that PUB12/PUB13 can be genetically manipulated to enhance the disease resistance in plants (Lu et al. 2011).”
• Plant pattern recognition receptor complexes at the plasma membrane. (2012, background): “Recently, an elegant study revealed the function of two E3ubiquitin ligases, PUB12 and PUB13, as negative regulators of FLS2 post-ligand perception [68 ].”